水稻根际木霉和杀菌剂防治水稻褐斑病的效果

Hage Kania, Narola Pongener, H. M. Devi, N. Ao, H. S. Devi
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摘要

水稻褐斑病在世界范围内普遍发生,对粮食产量造成大量的数量和质量损失。这种疾病是1942-1943年史诗般的“孟加拉大饥荒”的罪魁祸首,导致400万人死亡。研究了水稻根际木霉和杀菌剂对水稻褐斑病防治的效果。从水稻根际土壤中分离得到木霉菌株20株,采用双培养法进行筛选,筛选出抑菌力最强的菌株Ti20、Ti19和Ti16,分别为67.86%、64.29%和60.71%。采用毒食品法对六种杀菌剂(六康唑、丙环唑、多菌灵+代森锰锌、甲螨灵+代森锰锌、多菌灵和氯化铜)进行了抑菌效果评价,筛选出丙环唑、六环唑和氯化铜,并对所选木霉进行了配伍性试验。在盆栽试验中,选择木霉与杀菌剂(Ti19 + Hexaconazole 5% SC)配伍效果最好的木霉,采用种子处理、浸苗处理和叶面喷雾处理组合进行病害防治。盆栽试验结果表明,Ti19和Hexaconazole 5% SC处理在分蘖期、孕穗期和挤奶期的病害严重程度最低,分别为3.70%、7.41%和11.11%,产量为28.27 g/盆,显著高于对照。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of rice rhizospheric Trichoderma spp and fungicide for the management of brown spot disease of rice
Brown spot of rice caused by Bipolaris oryzae is of worldwide occurrence and is known to cause substantial quantitative and qualitative losses in grain yield. This disease was responsible for the epic 'Great Bengal Famine' in 1942-1943, resulting in the death of 4 million people. Studies were carried out to evaluate the effect of rice rhizospheric Trichoderma spp and fungicide for the management of brown spot disease of rice. A total of 20 isolates of Trichoderma spp were isolated from rice rhizospheric soil and screened by dual culture method in which three isolates Ti20, Ti19 and Ti16 showing maximum inhibition of 67.86%, 64.29% and 60.71% were selected for further test. Six fungicides viz., Hexaconazole, Propiconazole, Carbendazim + Mancozeb, Metalaxyl + Mancozeb, Carbendazim and Copper oxychloride were evaluated against B. oryzae by poison food technique, out of which Propiconazole, Hexaconazole and Copper oxychloride were selected and further tested for their compatibility with the selected Trichoderma spp was done. The most efficient and compatible Trichoderma sp with fungicide i.e., (Ti19 + Hexaconazole 5% SC) were selected for managing the disease in pot experiment with different treatment combinations i.e., seed treatment, seedling dip and foliar spray. Results under pot experiment showed that, seed treatment with Ti19 and Hexaconazole 5% SC recorded lowest disease severity of 3.70%, 7.41% and 11.11% at tillering, booting and milking stages respectively, with significantly higher yield of 28.27 g/pot as compared to untreated control pots.
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