抗黄脉花叶病γ辐照秋葵突变体与亲本遗传变异的评价

S. Surendran, P. Udayan
{"title":"抗黄脉花叶病γ辐照秋葵突变体与亲本遗传变异的评价","authors":"S. Surendran, P. Udayan","doi":"10.25081/cb.2020.v11.6114","DOIUrl":null,"url":null,"abstract":"Okra (Abelmoschus esculentus (L.) Moench is a member of the Malvaceae family. It has been cultivated as one of the most important fruit vegetables in various tropical countries. In okra, the Yellow Vein Mosaic Disease (YVMD) is caused by more than ten different viruses and the widespread incidence of disease transmitted by the vector Bemisia tabaci (Genn.) has highly affected the successful cultivation and production of the crop. Mutation breeding using radiations is one of the promising methods to get disease resistant elite cultivars of various crops. In this study, attempts were made to evaluate the genetic variability of gamma-ray irradiated mutant of okra. Four ISSR primers generated 26 amplified fragments. Of the 26 fragments, the lowest number of fragments (5) was produced by the primer ISSR12. Between the parent (P) and mutant (M4; mutant from fourth-generation) varieties of okra, primer ISSR07, primer ISSR16 and primer ISSR21 produced two monomorphic bands and three polymorphic bands (60% polymorphism) whereas primer ISSR12 produced one monomorphic band and three polymorphic bands (75% polymorphism). The mean percentage of polymorphism generated by four primers was 63.75%. Although the level of polymorphism exhibited by different primers varied from intermediate (60%) to high (75%), all primers could distinguish parent and gamma-irradiated mutant varieties of okra.","PeriodicalId":10828,"journal":{"name":"Current Botany","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of genetic variability between yellow vein mosaic disease resistant gamma-irradiated okra mutant and parent\",\"authors\":\"S. Surendran, P. Udayan\",\"doi\":\"10.25081/cb.2020.v11.6114\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Okra (Abelmoschus esculentus (L.) Moench is a member of the Malvaceae family. It has been cultivated as one of the most important fruit vegetables in various tropical countries. In okra, the Yellow Vein Mosaic Disease (YVMD) is caused by more than ten different viruses and the widespread incidence of disease transmitted by the vector Bemisia tabaci (Genn.) has highly affected the successful cultivation and production of the crop. Mutation breeding using radiations is one of the promising methods to get disease resistant elite cultivars of various crops. In this study, attempts were made to evaluate the genetic variability of gamma-ray irradiated mutant of okra. Four ISSR primers generated 26 amplified fragments. Of the 26 fragments, the lowest number of fragments (5) was produced by the primer ISSR12. Between the parent (P) and mutant (M4; mutant from fourth-generation) varieties of okra, primer ISSR07, primer ISSR16 and primer ISSR21 produced two monomorphic bands and three polymorphic bands (60% polymorphism) whereas primer ISSR12 produced one monomorphic band and three polymorphic bands (75% polymorphism). The mean percentage of polymorphism generated by four primers was 63.75%. Although the level of polymorphism exhibited by different primers varied from intermediate (60%) to high (75%), all primers could distinguish parent and gamma-irradiated mutant varieties of okra.\",\"PeriodicalId\":10828,\"journal\":{\"name\":\"Current Botany\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-05-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Botany\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.25081/cb.2020.v11.6114\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Botany","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.25081/cb.2020.v11.6114","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

秋葵(秋葵)Moench是malvacae家族的一员。在许多热带国家,它已被栽培为最重要的水果蔬菜之一。秋葵黄脉花叶病(YVMD)是由十多种不同的病毒引起的,媒介烟粉虱(Genn.)传播疾病的广泛发生严重影响了作物的成功种植和生产。利用辐射诱变育种是获得作物抗病优良品种的一种很有前途的方法。在本研究中,试图评估秋葵γ射线辐照突变体的遗传变异性。4条ISSR引物产生26个扩增片段。在26个片段中,引物ISSR12产生的片段数量最少(5个)。在亲本(P)和突变体(M4)之间;引物ISSR07、ISSR16和ISSR21产生2条单态带和3条多态带(多态性为60%),引物ISSR12产生1条单态带和3条多态带(多态性为75%)。4个引物产生的多态性平均百分比为63.75%。虽然不同引物表现出的多态性水平从中等(60%)到高(75%)不等,但所有引物都能区分秋葵亲本和γ辐照突变品种。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of genetic variability between yellow vein mosaic disease resistant gamma-irradiated okra mutant and parent
Okra (Abelmoschus esculentus (L.) Moench is a member of the Malvaceae family. It has been cultivated as one of the most important fruit vegetables in various tropical countries. In okra, the Yellow Vein Mosaic Disease (YVMD) is caused by more than ten different viruses and the widespread incidence of disease transmitted by the vector Bemisia tabaci (Genn.) has highly affected the successful cultivation and production of the crop. Mutation breeding using radiations is one of the promising methods to get disease resistant elite cultivars of various crops. In this study, attempts were made to evaluate the genetic variability of gamma-ray irradiated mutant of okra. Four ISSR primers generated 26 amplified fragments. Of the 26 fragments, the lowest number of fragments (5) was produced by the primer ISSR12. Between the parent (P) and mutant (M4; mutant from fourth-generation) varieties of okra, primer ISSR07, primer ISSR16 and primer ISSR21 produced two monomorphic bands and three polymorphic bands (60% polymorphism) whereas primer ISSR12 produced one monomorphic band and three polymorphic bands (75% polymorphism). The mean percentage of polymorphism generated by four primers was 63.75%. Although the level of polymorphism exhibited by different primers varied from intermediate (60%) to high (75%), all primers could distinguish parent and gamma-irradiated mutant varieties of okra.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信