hplc法测定盐酸阿那非达泊西汀片剂剂型的分析方法验证

Dhwani A. Shah, Kunjal L. Vegad, E. D. Patel, Hitesh K. Prajapati, Ronak N. Patel, Y. K. Patel
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引用次数: 6

摘要

目的:建立一种简便、特异、准确、精密度高的RP-HPTLC方法,用于同时测定阿那非和达泊西汀的含量。方法:以氯仿:甲醇:乙酸乙酯:冰醋酸(5:2:3:2 .2,v/v/v/v)为流动相,在279 nm处进行色谱分离。结果:阿瓦那非和达泊西汀的RP-HPLC相关系数分别为0.9987和0.9991,线性范围分别为1040 ~ 3640 ng*spot-1和80 ~ 280 ng*spot-1。结论:所建立的方法可成功地应用于市售片剂剂型,结果具有较高的可信度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analytical method validation for estimation of avanafil and dapoxetine hydrochloride tablet dosage form by HPTLC method
Objective: A simple, specific, accurate and precise RP-HPTLC method has been developed and validated for simultaneous estimation of Avanafil and Dapoxetine. Methods: The chromatographic separation was achieved on Aluminium plates precoated with Silica gel 60 F254 using chloroform: methanol: ethyl acetate: glacial acetic acid (5:2:3:0.2, v/v/v/v) as mobile phase detected at 279 nm. Results: The correlation coefficient for RP-HPLC method was found to be 0.9987 for Avanafil and 0.9991 Dapoxetine and the linearity range was found to be 1040-3640 ng*spot-1 for Avanafil and 80-280 ng*spot-1 for Dapoxetine. Conclusions: The developed method was successfully applied to marketed tablet dosage form and the results were found with higher confidence.
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