Dhwani A. Shah, Kunjal L. Vegad, E. D. Patel, Hitesh K. Prajapati, Ronak N. Patel, Y. K. Patel
{"title":"hplc法测定盐酸阿那非达泊西汀片剂剂型的分析方法验证","authors":"Dhwani A. Shah, Kunjal L. Vegad, E. D. Patel, Hitesh K. Prajapati, Ronak N. Patel, Y. K. Patel","doi":"10.26510/2394-0859.PBE.2017.26","DOIUrl":null,"url":null,"abstract":"Objective: A simple, specific, accurate and precise RP-HPTLC method has been developed and validated for simultaneous estimation of Avanafil and Dapoxetine. Methods: The chromatographic separation was achieved on Aluminium plates precoated with Silica gel 60 F254 using chloroform: methanol: ethyl acetate: glacial acetic acid (5:2:3:0.2, v/v/v/v) as mobile phase detected at 279 nm. Results: The correlation coefficient for RP-HPLC method was found to be 0.9987 for Avanafil and 0.9991 Dapoxetine and the linearity range was found to be 1040-3640 ng*spot-1 for Avanafil and 80-280 ng*spot-1 for Dapoxetine. Conclusions: The developed method was successfully applied to marketed tablet dosage form and the results were found with higher confidence.","PeriodicalId":19998,"journal":{"name":"Pharmaceutical and Biological Evaluations","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"Analytical method validation for estimation of avanafil and dapoxetine hydrochloride tablet dosage form by HPTLC method\",\"authors\":\"Dhwani A. Shah, Kunjal L. Vegad, E. D. Patel, Hitesh K. Prajapati, Ronak N. Patel, Y. K. Patel\",\"doi\":\"10.26510/2394-0859.PBE.2017.26\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: A simple, specific, accurate and precise RP-HPTLC method has been developed and validated for simultaneous estimation of Avanafil and Dapoxetine. Methods: The chromatographic separation was achieved on Aluminium plates precoated with Silica gel 60 F254 using chloroform: methanol: ethyl acetate: glacial acetic acid (5:2:3:0.2, v/v/v/v) as mobile phase detected at 279 nm. Results: The correlation coefficient for RP-HPLC method was found to be 0.9987 for Avanafil and 0.9991 Dapoxetine and the linearity range was found to be 1040-3640 ng*spot-1 for Avanafil and 80-280 ng*spot-1 for Dapoxetine. Conclusions: The developed method was successfully applied to marketed tablet dosage form and the results were found with higher confidence.\",\"PeriodicalId\":19998,\"journal\":{\"name\":\"Pharmaceutical and Biological Evaluations\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmaceutical and Biological Evaluations\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.26510/2394-0859.PBE.2017.26\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmaceutical and Biological Evaluations","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26510/2394-0859.PBE.2017.26","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Analytical method validation for estimation of avanafil and dapoxetine hydrochloride tablet dosage form by HPTLC method
Objective: A simple, specific, accurate and precise RP-HPTLC method has been developed and validated for simultaneous estimation of Avanafil and Dapoxetine. Methods: The chromatographic separation was achieved on Aluminium plates precoated with Silica gel 60 F254 using chloroform: methanol: ethyl acetate: glacial acetic acid (5:2:3:0.2, v/v/v/v) as mobile phase detected at 279 nm. Results: The correlation coefficient for RP-HPLC method was found to be 0.9987 for Avanafil and 0.9991 Dapoxetine and the linearity range was found to be 1040-3640 ng*spot-1 for Avanafil and 80-280 ng*spot-1 for Dapoxetine. Conclusions: The developed method was successfully applied to marketed tablet dosage form and the results were found with higher confidence.