人脐带源性冻干组织工程基质的生物学和功能特性

A. A. Kondratenko, L. Kalyuzhnaya, D. Tovpeko, V. S. Sheveleva, R. Glushakov
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引用次数: 1

摘要

使用来自脱细胞细胞外基质(dECM)的组织工程产品(TEP)治疗深层皮肤病变是一种促进再生愈合的组织工程方法。无细胞制剂再现了组织的层次复杂性,模拟了吸引细胞所必需的结构、生化和机械信号,并且是生物活性分子的来源。人类脐带生物材料具有胚胎外起源的胎儿表型,因此是可用的,并且在其使用中没有伦理限制。基洛夫军事医学学院的组织工程实验室从高度再生的人类脐带中开发了一种TEP,并申请了专利,该TEP以基质和水凝胶基质的形式存在。为了研究其再生潜力,将组织工程固体和水凝胶基质冻干液植入小型猪全层伤口周围。分析了炎症反应的外部征象和冻干植入区活检标本的组织学图像。研究了用两种基质的冻干物“调理”的营养培养基对从迷你猪皮肤中分离的成纤维细胞样细胞的活力和迁移活性的影响。体外和体内实验表明基质冻干细胞具有良好的生物相容性和生物活性。与对照相比,植入样品促进了成熟表皮的更快形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biological and functional properties of human umbilical cord-derived lyophilized tissue-engineered matrices
The use of tissue-engineered products (TEP) from decellularized extracellular matrix (dECM) to treat deep skin lesions is a tissue engineering method that promotes regenerative healing. Cell-free preparations reproduce the hierarchical complexity of tissues, mimic structural, biochemical and mechanical signals that are necessary to attract cells, and are a source of bioactive molecules. The human umbilical cord biomaterial has a fetal phenotype with extra-embryonic origin, and therefore is available and has no ethical limitations in its use. The tissue engineering laboratory at Kirov Military Medical Academy developed and patented a TEP from the highly regenerative human umbilical cord in the form of matrix and hydrogel matrix. To study its regenerative potential, lyophilisates of tissue-engineered solid-state and hydrogel matrices were implanted around mini pig fullthickness wounds in vivo. The external signs of inflammatory response and the histological images of biopsy specimens from the lyophilizate implantation areas were analyzed. The effect of nutrient media, «conditioned» with lyophilizates of both matrices, on the viability and migration activity of fibroblast-like cells, isolated from mini pig skin, was investigated. The matrix lyophilisates showed good biocompatibility and bioactivity in in vitro and in vivo experiments. Implantation of the samples promoted faster formation of mature epidermis compared to the control.
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