单性黄瓜(Cucumis sativus L.)遗传多样性和群体结构的SSR分析

IF 0.2 Q4 HORTICULTURE
Manpreet Kaur, Parveen Sharma, Akhilesh Sharma, Hem Lata, N. Kumar
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引用次数: 0

摘要

利用SSR (simple sequence repeats)标记对14个单性黄瓜(Cucumis sativus L.)基因型的遗传多样性和群体关系进行了分析。在本研究中,59个SSR标记全面显示了黄瓜基因型间的多态性。共鉴定出252个等位基因,平均每个位点有4.27个等位基因,引物的多态性信息含量(PIC)在0.34 ~ 0.84之间,平均值为0.62。主要等位基因频率和杂合度分别为0.21 ~ 0.75和0.43 ~ 0.89。引物Cs- Female-4的主等位基因频率最高,而引物SSR11742的多态信息含量最高。树状图将基因型分为A组和B组,分别有8个和6个基因型。Jaccard相似系数在0.63 ~ 0.86之间,DDPCG3与PLP-1基因型相似性最大,DDPCG8与PLP Gy-1-08B基因型相似性最小。种群结构表现为3个亚种群,并有一定的混杂。SSR标记主坐标分析(PCoA)表明,2个样地的基因型在2个轴上分布均匀,累积变异率为41.76%。黄瓜本地基因型的遗传差异为黄瓜的基因型鉴定、基因定位和克隆提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SSR analysis to assess genetic diversity and population structure in parthenocarpy cucumber (Cucumis sativus L.)
The genetic diversity and population relationship was determined in 14 genotypes of parthenocarpic cucumber (Cucumis sativus L.) using simple sequence repeats (SSR) markers. In this study, fifty-nine SSR markers comprehensively showed polymorphism among cucumber genotypes. Total 252 alleles were identified with an average of 4.27 alleles per locus, while the polymorphism information content (PIC) of the primers ranged from 0.34 to 0.84 with a mean value of 0.62. The major allele frequency and heterozygosity ranged from 0.21 to 0.75 and from 0.43 to 0.89, respectively. Maximum major allele frequency was reported with primer Cs- Female-4, whereas the maximum value of polymorphic information content was found with the primer SSR11742. The dendrogram clustered genotypes into two main groups A and B with 8 and 6 genotypes, respectively. Jaccard’s similarity coefficient ranged from 0.63 to 0.86 with maximum similarity between genotypes DDPCG3 and PLP-1, whereas minimum similarity was observed between DDPCG8 and PLP Gy-1-08B. The population structure revealed three sub-populations with some admixtures. Principal coordinate analysis (PCoA) with SSR markers revealed that the genotypes were uniformly distributed across the two axes in both the plots with 41.76% of cumulative variation. The genetic divergence within indigenous genotypes allow genotypic identification, gene mapping and cloning for improvement in cucumber breeding.
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来源期刊
Journal of Horticultural Sciences
Journal of Horticultural Sciences Agricultural and Biological Sciences-Plant Science
CiteScore
0.30
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