肾上皮细胞中钠/硫酸钠共转运的激素调节。

H. J. Lee, K. Sagawa, W. Shi, H. Murer, M. Morris
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引用次数: 11

摘要

婴儿、幼儿和孕妇血清硫酸根浓度升高,至少部分原因是由于肾脏硫酸根重吸收增加。关于激素,特别是生长、发育和妊娠过程中激素对肾脏硫酸盐重吸收的影响,我们所知甚少。本研究的目的是研究生长激素(GH)、胰岛素样生长因子1 (IGF-1)、黄体酮(PG)和17 -雌二醇(EST)对肾脏钠/硫酸钠共转运的影响。测定了Madin-Darby犬肾(MDCK)/NaSi-1细胞(MDCK细胞稳定转染了大鼠钠/硫酸盐共转运体(NaSi-1) cDNA)和负鼠肾(OK)细胞对35s -硫酸盐的摄取。RT-PCR检测NaSi-1 mRNA表达,ELISA检测NaSi-1蛋白表达水平。GH (0.1 nM)可显著增加MDCK/NaSi-1细胞的钠/硫酸盐共转运,最高可达35%。IGF-1诱导了钠/硫酸盐共转运的浓度相关刺激,在1000 nM处观察到最大反应(增加59%)。当细胞与10 nM PG、10 nM EST或10 nM PG/10 nM EST预孵育时,钠依赖性硫酸盐摄取显著增加,分别达到41%、46%或39%。OK细胞表现出内源性钠依赖性硫酸盐运输;在与GH、IGF-1和PG/EST预孵育的OK细胞中也观察到显著增加的钠/硫酸盐共转运,尽管没有单独与EST孵育。与对照相比,0.1 nM GH、100 nM IGF-1、10 nM PG和/或10 nM EST处理MDCK/NaSi-1细胞中NaSi-1 mRNA和NaSi-1蛋白水平显著升高。这些结果表明,新生儿、青少年、孕妇和动物肾脏硫酸盐重吸收的增加可能是由较高的血浆GH、IGF-1或PG/EST浓度所产生的NaSi-1 mRNA稳态水平的增加所介导的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hormonal regulation of sodium/sulfate co-transport in renal epithelial cells.
Serum sulfate concentrations are elevated in infants, young children, and pregnant women due, at least in part, to increased renal sulfate reabsorption. Little is known about the effects of hormones, particularly those involved in growth, development, and pregnancy, on renal sulfate reabsorption. The objective of this investigation was to examine the effects of growth hormone (GH), insulin-like growth factor 1 (IGF-1), progesterone (PG), and 17beta-estradiol (EST) on renal sodium/sulfate co-transport. 35S-sulfate uptake was determined in Madin-Darby canine kidney (MDCK)/NaSi-1 cells (MDCK cells that have been stably transfected with rat sodium/sulfate co-transporter (NaSi-1) cDNA) and in opossum kidney (OK) cells. NaSi-1 mRNA was determined by RT-PCR and protein levels by ELISA. GH (0.1 nM) significantly increased the sodium/sulfate co-transport in MDCK/NaSi-1 cells up to 35%. IGF-1 induced a concentration-related stimulation of the sodium/sulfate co-transport with a maximal response observed at 1000 nM (59% increase). Sodium-dependent sulfate uptake was significantly increased when cells were preincubated with 10 nM PG, 10 nM EST, or 10 nM PG/10 nM EST up to 41%, 46%, or 39%, respectively. OK cells exhibited endogenous sodium-dependent sulfate transport; significantly increased sodium/sulfate co-transport was also observed in OK cells that were preincubated with GH, IGF-1, and PG/EST, although not with EST alone. The NaSi-1 mRNA and NaSi-1 protein levels were significantly increased in MDCK/NaSi-1 cells treated with 0.1 nM GH, 100 nM IGF-1, 10 nM PG, and/or 10 nM EST compared with control. These results suggest that the increased renal sulfate reabsorption that occurs in neonates, young and pregnant humans, and animals could be mediated by the increased steady-state levels of NaSi-1 mRNA produced by the higher plasma concentrations of GH, IGF-1, or PG/EST.
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