微藻中强抗氧化剂类胡萝卜素虾青素的温和提取工艺优化

Q3 Chemical Engineering

Chemical engineering transactions Pub Date : 2021-07-01 DOI:10.3303/CET2187026

M. Chronis, Vasiliki Christopoulou, S. Papadaki, Marina Stramarkou, M. Krokida

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引用次数: 4

摘要

微藻是食品中生物活性化合物的重要来源。本研究从雨红球菌(H. pluvialis)微藻中提取多功能提取物，开发用于食品添加剂。总萃取物采用超声波辅助萃取，采用环保型和食品级溶剂回收。为了优化类胡萝卜素，特别是虾青素的提取，研究了不同超声强度下，5种不同比例(1/100 ~ 1/5 g生物质/ mL溶剂)的提取效果。采用高压液相色谱(HPLC-DAD)和紫外-可见光谱技术对提取的提取物进行分析。丙酮是提取类胡萝卜素最有效的溶剂。然而，d -柠檬烯和中链甘油三酯提取物也有令人满意的类胡萝卜素回收率。d -柠檬烯是首选的溶剂，因为含有大量类胡萝卜素的提取物被回收，允许它们直接用于食品中，避免进一步的冷凝步骤。

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Optimization of Mild Extraction Methods for the Efficient Recovery of Astaxanthin, a Strong Food Antioxidant Carotenoid from Microalgae

Microalgae represent an important source of bioactive compounds for food applications. In this study, multifunctional extracts from Haematococcus pluvialis (H. Pluvialis) microalga have been developed in order to be used as food additives. Total extracts were recovered by ultrasound assisted extraction using eco-friendly and food grade solvents. Five different ratios ranging from 1/100 to 1/5 g biomass/ mL solvent were studied in different ultrasound intensities in order to optimise the extraction of carotenoids, especially of astaxanthin. The produced extracts were analysed using High Pressure Liquid Chromatography (HPLC-DAD) and UV-Vis spectroscopy techniques. Acetone was the most efficient solvent for the extraction of carotenoids in all tested ratios. However, D-limonene and medium chain triglycerides extracts also gave a satisfying recovery of carotenoids. D-limonene was the preferred solvent since extracts with high amounts of carotenoids were recovered, permitting their direct use in food products and avoiding further condensation steps.

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来源期刊

Chemical engineering transactions Chemical Engineering-Chemical Engineering (all)

CiteScore

1.40

自引率

0.00%

发文量

审稿时长

6 weeks

期刊介绍： Chemical Engineering Transactions (CET) aims to be a leading international journal for publication of original research and review articles in chemical, process, and environmental engineering. CET begin in 2002 as a vehicle for publication of high-quality papers in chemical engineering, connected with leading international conferences. In 2014, CET opened a new era as an internationally-recognised journal. Articles containing original research results, covering any aspect from molecular phenomena through to industrial case studies and design, with a strong influence of chemical engineering methodologies and ethos are particularly welcome. We encourage state-of-the-art contributions relating to the future of industrial processing, sustainable design, as well as transdisciplinary research that goes beyond the conventional bounds of chemical engineering. Short reviews on hot topics, emerging technologies, and other areas of high interest should highlight unsolved challenges and provide clear directions for future research. The journal publishes periodically with approximately 6 volumes per year. Core topic areas: -Batch processing- Biotechnology- Circular economy and integration- Environmental engineering- Fluid flow and fluid mechanics- Green materials and processing- Heat and mass transfer- Innovation engineering- Life cycle analysis and optimisation- Modelling and simulation- Operations and supply chain management- Particle technology- Process dynamics, flexibility, and control- Process integration and design- Process intensification and optimisation- Process safety- Product development- Reaction engineering- Renewable energy- Separation processes- Smart industry, city, and agriculture- Sustainability- Systems engineering- Thermodynamic- Waste minimisation, processing and management- Water and wastewater engineering