激光诱导振荡微泡的分子传递和转染

The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2014-04-13 DOI:10.1109/NEMS.2014.6908813

Qihui Fan, Wenqi Hu, A. Ohta

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引用次数: 0

摘要

利用微秒激光脉冲诱导的振荡微泡，成功地将外源分子传递到哺乳动物的局部单贴壁细胞中。由于微泡振荡产生的剪切应力导致附近细胞的穿孔。通过调整激光位置，在目标细胞下方产生微泡，可以穿孔特定的单个细胞。通过递送500 kda的fitc -葡聚糖染料和16 kb的质粒，证明了局部单细胞的穿孔。

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Molecular delivery and transfection by laser-induced oscillating microbubbles

Exogenous molecules were successfully delivered into localized single mammalian adherent cells with oscillating microbubbles induced by microsecond laser pulses. The shear stress due to the oscillation of the microbubble causes the poration of a nearby cell. Specific single cells can be porated by adjusting the laser position to create the microbubbles beneath the target cell. The poration of localized single cells was demonstrated with the delivery of 500-kDa FITC-Dextran dye and 16-kb plasmids.

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The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

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