粪肠球菌噬菌体结构蛋白

R. Stevens, Hongming Zhang, Chaiwing Hsiao, S. Kachlany, Eduardo M. B. Tinoco, Jessica DePew, D. Fouts
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引用次数: 6

摘要

采用诱导法从粪肠球菌根管分离物中分离得到一个温性Siphoviridae噬菌体φEf11。序列分析表明,φEf11基因组包括一个连续的8个基因模块,其功能与头部结构组装有关,另一个由10个连续基因组成的模块,其产物负责尾部结构组装。对φEf11衍生物的病毒粒子进行SDS-PAGE分析,发现了11条解析良好的蛋白带。为了将从DNA序列数据推断出的功能基因定位与纯化病毒的结构蛋白分析相统一,对其中6条SDS-PAGE条带进行了质谱分析。质谱分析的6个蛋白带中有5个与φEf11基因组中鉴定的4个orf预测指定的氨基酸序列相同。这些包括:ORF8(预测支架蛋白),ORF10(预测主要头部蛋白),ORF15(预测主要尾部蛋白)和ORF23(推定抗受体)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structural proteins of Enterococcus faecalis bacteriophage ϕEf11
ABSTRACT φEf11, a temperate Siphoviridae bacteriophage, was isolated by induction from a root canal isolate of Enterococcus faecalis. Sequence analysis suggested that the φEf11 genome included a contiguous 8 gene module whose function was related to head structure assembly and another module of 10 contiguous genes whose products were responsible for tail structure assembly. SDS-PAGE analysis of virions of a φEf11 derivative revealed 11 well-resolved protein bands. To unify the deduced functional gene assignments emanating from the DNA sequence data, with the structural protein analysis of the purified virus, 6 of the SDS-PAGE bands were subjected to mass spectrometry analysis. 5 of the 6 protein bands analyzed by mass spectrometry displayed identical amino acid sequences to those predicted to be specified by 4 of the ORFs identified in the φEf11 genome. These included: ORF8 (predicted scaffold protein), ORF10 (predicted major head protein), ORF15 (predicted major tail protein), and ORF23 (presumptive antireceptor).
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