牛痘诺卡菌IMV B-7405表面活性剂与精油对酵母菌的协同作用

Q4 Biochemistry, Genetics and Molecular Biology
T. Pirog, L. Kliuchka, T. Shevchuk, F. Muchnyk
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引用次数: 0

摘要

念珠菌属代表的耐药菌株数量的增加,能够在各种表面形成生物膜,刺激寻找新的替代方法来对抗它们,其中之一是使用天然来源的化合物,如精油。然而,与此同时,它们的浓度应该是最低的,这是由于精油对人的中枢神经系统和吸入性肺炎造成严重损害的能力。这导致有必要寻找新的方法来降低精油的浓度,同时保持其特性,特别是通过与其他抗菌剂(可以是微生物表面活性剂)混合使用。此前研究发现,在牛痘诺卡菌IMV B-7405表面活性剂的作用下,酵母生物膜的破坏程度取决于生长底物的性质,在用纯化甘油合成的制剂存在时破坏程度最高。的目标。研究不同质量的甘油和精油合成的牛痘诺卡菌IMV B-7405表面活性剂的协同抑菌活性及其对生物膜的破坏作用。方法。牛痘乳杆菌IMV B-7405生长在含有浓度为2% (v/v)的纯化甘油或生物柴油生产废料的培养基中作为碳源。用改良的Folch混合液从培养液上清液中提取表面活性剂。用最小抑菌浓度指标测定了精油、表面活性剂及其混合物的抑菌活性。为了评估表面活性剂与精油混合物的协同作用,采用分数抑制浓度指数。生物膜破坏程度(%)以未经处理和经表面活性剂、精油或其混合孔处理的聚苯乙烯微孔板细胞粘附的差异来确定。结果。研究发现,牛痘奈希菌IMV B-7405在纯化甘油和生物柴油废液上合成的表面活性剂与肉桂和柠檬草精油混合后均具有协同抑菌活性。结果表明,纯化甘油与肉桂和柠檬草精油合成的表面活性剂对白色念珠菌D-6、效用念珠菌BVS-65和热带念珠菌RE-2的最低抑制浓度分别为1.8 ~ 7.5和3.7 ~ 15 μg/mL,低于单独使用表面活性剂(30 ~ 60 μg/mL)、肉桂或柠檬草精油(156 ~ 312 μg/mL)时的最低抑制浓度。使用从生物柴油生产废料中获得的表面活性剂和肉桂或柠檬草精油的混合物,可以将后者对所研究的酵母试验培养物的最低抑制浓度降低14-56倍。同时,分数抑制浓度指数均不超过0.5,说明这些化合物混合后的抗真菌活性具有协同作用。在纯化甘油和生物柴油生产废弃物中合成的表面活性剂与肉桂或柠檬草精油混合作用下,假丝酵母生物膜的破坏率分别达到60 - 67%和67-77%,比单独使用每种单一工艺平均高25-35%。结论。本文的研究结果证实了之前得到的数据,即在传统底物和有毒工业废物上合成的母牛乳杆菌IMV B-7405表面活性剂与精油具有抗菌和抗粘附的协同作用,这使我们可以将其视为对抗念珠菌属代表的所谓“抗真菌锁”的潜在成分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synergistic Effect of Surfactants of Nocardia vaccinii IMV B-7405 and Essential Oils on Candida Genus Yeast
The increase in the number of resistant strains of Candida genus representatives, capable of forming biofilms on various surfaces, stimulates the search for new, alternative methods of combating them, one of which is the use of compounds of natural origin, such as essential oils. However, at the same time, their concentration should be minimal, which is due to the ability of essential oils to cause severe damage of the human’s central nervous system and aspiration pneumonia. This leads to the necessity of searching for new methods to reduce the concentration of essential oils and at the same time to preserve their properties, in particular, by their use in a mixture with other antimicrobial agents, which can be microbial surfactants. Previously, it was found that the degree of yeast biofilm destruction under the action of Nocardia vaccinii IMV B-7405 surfactants dependson the nature of the growth substrate and is the highest in the presence of preparations synthesized on purified glycerol. Aim. To study the synergism of antifungal activity and the role in the destruction of biofilms of a mixture of Nocardia vaccinii IMV B-7405 surfactants synthesized on glycerol of different quality and essential oils. Methods. N. vaccinii IMV B-7405 was grown in a medium containing purified glycerol or waste from biodiesel production at a concentration of 2% (v/v) as carbon sources. The surfactants were extracted from the supernatant of cultural liquid by a modified Folch mixture. The antimicrobial activity of essential oils, surfactants, and their mixtures was determined by the index of the minimum inhibitory concentration. To assess the synergistic effect of a mixture of surfactants with essential oils, the fractional inhibitory concentration index was used. The degree of biofilm destruction (%) was determined as the difference between the cell adhesion in untreated and treated with surfactants, essential oil, or their mixture wells of the polystyrene microplates. Results. It was found that the surfactants synthesized by N. vaccinii IMV B-7405 on both purified glycerol and waste from biodiesel production showed synergistic antifungal activity in mixtures with cinnamon and lemongrass essential oils. Thus, the minimum inhibitory concentrations against Candida albicans D-6, Candida utilis BVS-65, and Candida tropicalis RE-2 of a mixture of surfactants synthesized on purified glycerol with cinnamon and lemongrass essential oils were 1.8—7.5 and 3.7— 15 μg/mL, respectively, and were lower than in the case of using surfactants (30-60 μg/mL), cinnamon or lemongrass essential oil (156—312 μg/mL) alone. The use of a mixture of surfactants obtained on waste from biodiesel production and cinnamon or lemongrass essential oils made it possible to reduce the minimum inhibitory concentrations of the latter against studied yeast test cultures by 14—56 times. At the same time, the index of fractional inhibitory concentration did not exceed 0.5, which indicates the synergism of the antifungal activity of the mixture of these compounds. The destruction of Candida yeast biofilms under the action of surfactants synthesized on both purified glycerol and waste from biodiesel production in a mixture with cinnamon or lemongrass essential oils reached 60—67 and 67—77%, respectively, which is an average of 25—35% higher compared to the use of each monopreparation separately. Conclusions. The results presented in this paper confirm the previously obtained data that N. vaccinii IMV B-7405 surfactants, synthesized on both traditional substrates and toxic industrial wastes, have antimicrobial and antiadhesive synergistic action with essential oils, which allows us to consider them as potential components of the so-called «antifungal locks» in the fight against of Candida genus representatives.
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Mikrobiolohichnyi zhurnal
Mikrobiolohichnyi zhurnal Medicine-Microbiology (medical)
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