甲状腺乳头状癌和多结节性甲状腺肿患者Vaspin基因多态性与血清Vaspin水平的关系

Sahar Torki, M. Nezhadali, M. Hedayati
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摘要

背景与目的:Vaspin是一种新型的脂肪细胞因子。各种研究表明,vaspin与许多类型的癌症有关。本研究旨在探讨vaspin rs2236242基因多态性与女性乳头状甲状腺癌(PTC)和多结节性甲状腺肿(MNG)患者vaspin水平的关系。方法:在本病例对照研究中,参与者为134名女性。他们被分成三组;PTC (n=49)、MNG (n=30)和对照组(n=55)。参与者外周血10毫升,5毫升用于DNA提取,5毫升用于血清制备。采用Sandwich ELISA试剂盒检测vaspin水平。vaspin rs2236242多态性基因型检测采用四引物扩增难解突变系统/聚合酶链反应法。结果:三组患者血清vaspin水平差异无统计学意义。各组vaspin rs2236242多态性基因型与血清vaspin水平无显著相关性。Logistic回归分析显示,MNG组rs2236242多态性基因型频率与对照组比较差异有统计学意义。TA基因型对PTC和MNG均有保护作用(P<0.05)。结论:PTC组、MNG组与对照组血清vaspin水平无明显差异。rs2236242基因多态性与血清vaspin水平无相关性。rs2236242的TA基因型对MNG具有保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association Between Vaspin Gene Polymorphism and Serum Vaspin Level in Women With Papillary Thyroid Cancer and Multinodular Goiter
Background and Objectives: Vaspin is a novel adipocytokine. Various studies have shown that vaspin is associated with many types of cancer. This study aims to determine the association of vaspin rs2236242 gene polymorphism with vaspin level in women with papillary thyroid cancer (PTC) and Multinodular goiter (MNG). Methods: In this case-control study participants were 134 women. They were divided into three groups; PTC (n=49), MNG (n=30) and control (n=55). Ten milliliter peripheral blood samples were taken from participants, 5 ml was used for DNA extraction and 5 ml for preparation of serum. The vaspin level was measured by Sandwich ELISA kit. The genotype determination of vaspin rs2236242 polymorphism was done using the tetra primer-amplification refractory mutation system/polymerase chain reaction method. Results: There were no significant difference in serum vaspin level among the three groups. There was no significant association between genotypes of vaspin rs2236242 polymorphism and serum vaspin level in any groups. Logistic regression analysis showed that the difference in the frequency of the genotypes of rs2236242 polymorphism in the MNG group was significant compared to the control group. The TA genotype showed a protective effect against PTC and MNG (P<0.05). Conclusion: There is no significant difference in vaspin level between PTC, MNG and control groups. There is no association between rs2236242 gene polymorphism and serum vaspin level. The TA genotype of rs2236242 has a protective effect against MNG.
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