利用微aut- am和Sensititre Yeast-One评价念珠菌体外敏感性的比较研究

IF 2.1 Q3 MICROBIOLOGY

Microbiology Research Pub Date : 2023-08-07 DOI:10.3390/microbiolres14030072

L. Trovato, Maddalena Calvo, G. Scalia, S. Oliveri

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引用次数: 1

摘要

背景。在侵袭性真菌感染病原菌中，念珠菌是最常见的病原。严重感染率的上升和抗菌素耐药性的出现突出了体外药敏试验的重要性。EUCAST和CLSI已经建立了可靠但难以在实验室常规中应用的参考微稀释。商业微稀释可能是诊断工作流程中有价值的替代方案。方法。从阳性血液样本中同时采集50个念珠菌，分别采用Sensititre Yeast-One微量稀释作为标准药敏试验和Micronaut-AM作为实验方法。对这两种技术进行了比较，评估了与广泛巩固的Sensititre Yeast-One相比，Micronaut-AM的有效性。结果。这两种技术显示出最佳的一致性率，证实了商业微量稀释试剂盒在诊断工作流程中的可靠性。结果显示敏感和耐药菌株具有显著的一致性，突出了不同鉴定的念珠菌种类的轻微差异。结论。今后应进一步开展抗真菌药敏试验研究，包括对不同念珠菌种类可能的耐药表型的分子确证和扩大分离株数量。此外，在检验的商业微量稀释方法执行后，计划临床试验将是有趣的。

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A Comparative Prospective Study in Evaluating Candida spp. In Vitro Susceptibility through Micronaut-AM and Sensititre Yeast-One

Background. Among invasive fungal infection pathogens, Candida spp. represent the most common aetiological agents. The increasing rate of severe infections and the emergence of antimicrobial resistance highlight the importance of in vitro susceptibility testing. The EUCAST and the CLSI have established reference microdilutions that are reliable but difficult to apply in a laboratory routine. Commercial microdilutions could represent a valuable alternative within a diagnostic workflow. Methods. A number of 50 Candida spp. collected from positive blood samples simultaneously underwent the Sensititre Yeast-One microdilution as a standard susceptibility test and the Micronaut-AM as an experimental method. A comparison between the two techniques was produced, evaluating the effectiveness of the Micronaut-AM compared to the extensively consolidated Sensititre Yeast-One. Results. The two techniques revealed optimal agreement rates, confirming the reliability of the commercial microdilution kits within the diagnostic workflows. The results showed remarkable concordance for both susceptible and resistant isolates, highlighting slight variations in the different identified Candida species. Conclusions. Future studies about antifungal susceptibility testing should be encouraged, including molecular confirmation of possible resistance phenotypes and extended isolate numbers for the different Candida species. Moreover, it would be interesting to plan clinical trials after the execution of the examined commercial microdilution methods.

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来源期刊

Microbiology Research MICROBIOLOGY-

CiteScore

1.90

自引率

6.70%

发文量

审稿时长

10 weeks

期刊介绍： Microbiology Research is an international, online-only, open access peer-reviewed journal which publishes original research, review articles, editorials, perspectives, case reports and brief reports to benefit researchers, microbiologists, physicians, veterinarians. Microbiology Research publishes ‘Clinic’ and ‘Research’ papers divided into two different skill and proficiency levels: ‘Junior’ and ‘Professional’. The aim of this four quadrant grid is to encourage younger researchers, physicians and veterinarians to submit their results even if their studies encompass just a limited set of observations or rely on basic statistical approach, yet upholding the customary sound approach of every scientific article.