肽标记用水溶性五甲基吲哚菁染料的合成与表征

ECSOC-25 Pub Date : 2022-07-18 DOI:10.3390/ecsoc-25-11768

Cátia D. F. Martins, M. Raposo, S. Costa

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引用次数: 2

摘要

合成了一种水溶性五胺菁染料，用于与多肽的进一步偶联。染料的制备采用液相化学方法完成，合成的化合物采用核磁共振、紫外-可见吸收、荧光光谱和质谱等常用技术进行表征。研究了花青素4在pH为7.4的磷酸盐缓冲盐水(PBS)水溶液中的光物理性质。巯基羧酸4在646 nm处具有较窄的吸收带和较高的摩尔消光系数(log ε = 5.11)。在近红外(NIR)区发现最大荧光波长为661 nm。因此，由于染料4具有优异的光谱特性、良好的水溶性和近红外发射特性，可用于合成荧光共振能量转移(FRET)探针，用于体外酶解研究。

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Synthesis and Characterization of a Water-Soluble Pentamethine Indocyanine Dye for Peptide Labeling

: A water-soluble pentamethine cyanine dye was synthesized for further conjugation with peptides. The dye preparation was accomplished by solution-phase chemistry and the synthesized compounds were characterized by the usual techniques (nuclear magnetic resonance, UV-vis absorption and ﬂuorescence spectroscopies and mass spectrometry). The photophysical properties of cyanine 4 were studied in aqueous media, namely in phosphate-buffered saline (PBS) at pH 7.4. Sulfo-Cy5 carboxylic acid 4 exhibited a narrow absorption band and a high molar extinction coefﬁcient (log ε = 5.11) at 646 nm. The wavelength of maximum ﬂuorescence was found in the near-infrared (NIR) region at 661 nm. Therefore, due to its excellent spectral proprieties, good water solubility and emission in the NIR region, dye 4 can be employed in the synthesis of ﬂuorescence resonance energy transfer (FRET) probes for studying enzymatic hydrolysis in vitro.

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ECSOC-25

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