gaeumannomyes - phialophora复合体的线粒体质粒及其引物原位荧光标记检测

Joan M. Henson, The Can Caesar-TonThat
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引用次数: 2

摘要

Henson, j.m.和Caesar-TonThat, t.c. 1995。gaeumannomyes - phialophora复合体的线粒体质粒及其引物原位荧光标记检测。真菌学通报,2009,33 - 34。双链线状DNA线粒体质粒在大多数小麦革芽孢杆菌和小麦革芽孢杆菌分离株中检测到,但在革芽孢杆菌中检测到的频率较低,在其他革芽孢杆菌和Magnaporthe种中仅检测到1次。质粒长度为4-11 kb,在其5 '端明显被阻断,因为它们抵抗5 '→3 '外切酶酶切。对两个具有同源性的质粒进行了进一步的表征。显然,它们不是来自线粒体或核基因组,因为它们没有与它们强烈杂交。从一个质粒中克隆一个内部片段,并用冷冻切片的菌丝作为引物进行引物原位标记。携带质粒的菌株在质粒的线粒体位置有荧光信号,而不携带质粒的菌株没有荧光。这是首次在真菌中使用冷冻切片菌丝和线粒体探针进行原位荧光标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mitochondrial Plasmids of the Gaeumannomyces-Phialophora Complex and Their Detection by Primed, in Situ Fluorescence Labeling

Henson, J. M., and Caesar-TonThat, T. C. 1995. Mitochondrial plasmids of the Gaeumannomyces-Phialophora complex and their detection by primed, in situ fluorescence labeling. Experimental Mycology 19, 263-274. Double-stranded, linear DNA mitochondrial plasmids were detected in most Gaeumannomyces graminis var. tritici and var. avenae isolates, but were infrequently detected in G. graminis var. graminis , and only detected once in other Gaeumannomyces or Magnaporthe species. Plasmids were 4-11 kb and were apparently blocked at their 5′ termini, as they were resistant to 5′ → 3′ exonuclease digestion. Two plasmids that shared homology were further characterized. Apparently they were not derived from the mitochondrial or nuclear genomes as they did not hybridize strongly to them. An internal fragment was cloned from one plasmid and used as a primer for primed, in situ labeling with cryosectioned hyphae. Plasmid-bearing strains had fluorescent signal in a pattern expected of a mitochondrial location for plasmids, whereas plasmid-less strains did not fluoresce. This is the first use of cryosectioned hyphae and mitochondrial probes with in situ fluorescence labeling in fungi.

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