乙醛刺激正常和无烟香烟烟提取物处理的气道上皮细胞PKC活性。

T. Wyatt, S. Schmidt, S. Rennard, D. Tuma, J. Sisson
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引用次数: 68

摘要

在此之前,我们发现香烟烟雾中的挥发性成分乙醛刺激蛋白激酶C (PKC)途径并抑制纤毛运动。现在有一种“无烟”香烟(Eclipse),其中大部分烟草不燃烧,减少了提取物中的热解成分。我们假设乙醛是香烟烟雾中激活气道上皮细胞PKC的成分,因此Eclipse香烟不会激活上皮细胞PKC。在本研究中,牛支气管上皮细胞(BBEC)与香烟烟雾提取物(CSE)或Eclipse烟雾提取物(ESE)孵育。我们发现,暴露于5% CSE的细胞的PKC活性明显高于暴露于5% ESE或培养基的细胞。当用气相色谱法测量两种提取物的乙醛水平时,发现CSE的乙醛浓度(微米)比ESE高15-20倍。当BBEC用5%的CSE治疗时,纤毛搏动从基线水平进一步降低。在用ESE处理的细胞中没有观察到纤毛跳动的减少,这表明CSE中含有的乙醛减缓了纤毛。这些结果表明,香烟烟雾中的乙醛等挥发性成分可能通过pkc依赖机制抑制纤毛运动。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Acetaldehyde-stimulated PKC activity in airway epithelial cells treated with smoke extract from normal and smokeless cigarettes.
Previously, we have found that acetaldehyde, a volatile component of cigarette smoke, stimulates the protein kinase C (PKC) pathway and inhibits ciliary motility. A "smokeless" cigarette (Eclipse) now exists in which most of the tobacco is not burned, reducing the pyrolyzed components in the extract. We hypothesized that acetaldehyde is a component of cigarette smoke that activates PKC in the airway epithelial cell, and therefore the Eclipse cigarette would not activate epithelial cell PKC. In this study, bovine bronchial epithelial cells (BBEC) were incubated with cigarette smoke extract (CSE) or Eclipse smoke extract (ESE). We found that PKC activity was significantly higher in cells exposed to 5% CSE than cells exposed to 5% ESE or media. When acetaldehyde levels of both extracts were measured by gas chromatography, CSE was found to have 15-20 times greater concentration (microM) of acetaldehyde than ESE. When BBEC were treated with 5% CSE, ciliary beating was further decreased from baseline levels. This decrease in ciliary beating was not observed in cells treated with ESE, suggesting that acetaldehyde contained in CSE slows cilia. These results suggest that volatile components such as acetaldehyde in cigarette smoke may inhibit ciliary motility via a PKC-dependent mechanism.
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