通过确定病毒浓度比和miRNA表达谱来表征不同glav -3变异感染

D. Aldrich, R. Bester, J. Burger, H. J. Maree
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引用次数: 0

摘要

葡萄叶卷病(GLD)存在于世界上所有葡萄种植区,被认为是最严重的葡萄病毒性疾病。葡萄叶卷相关病毒3 (glav -3)被认为是GLD的主要原因,在南非葡萄园中已经确认了5个遗传变异群(I、II、III、VI和VII)。glav -3变体之间的生物学差异尚未得到充分证实。通过表征glrav3感染植物中的病毒浓度和应激反应性microRNA表达,本研究旨在更好地了解glrav3变体之间可能的生物学差异。在温室和田间条件下,采用定量反转录PCR法测定glav -3阳性和阴性葡萄的病毒浓度比(VCR)和miRNA含量。本研究发现,不同glav -3变异单感染植株的vcr存在统计学上的显著差异。有趣的是,尽管植株年龄、glav -3感染持续时间、接穗/砧木组合和生长条件存在显著差异,但数据集之间的平均vcr没有差异。一些mirna在感染和健康样本之间表现出统计学上显著的表达调节。数据集之间的miRNA表达差异很大,在glav -3感染较多的植物中观察到更大的总体miRNA反应。数据集之间的平均vcr没有显著差异,加上植物中某些mirna的一致调制可能已被感染较长时间,这是一个有希望的结果。这一发现可能表明植物防御机制成功地抑制了病毒的进一步复制,并且这些mirna与这种反应有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterisation of different GLRaV-3 variant infections by determining virus concentration ratios and miRNA expression profiles
Grapevine leafroll disease (GLD) is present in all grape-growing regions of the world and is considered the most significant grapevine viral disease. Grapevine leafroll-associated virus 3 (GLRaV-3) is considered the primary cause of GLD and in South African vineyards five genetic variant groups (I, II, III, VI and VII) have been confirmed. Biological distinctions between GLRaV-3 variants have not been fully validated. By characterising virus concentration and stress-responsive microRNA expression in GLRaV-3 infected plants, this study aimed to glean a better understanding of the possible biological distinctions between GLRaV-3 variants. Quantitative reverse transcription PCR was utilised for virus concentration ratio (VCR) determination and miRNA quantitation in GLRaV-3 positive and negative grapevines grown under greenhouse and field conditions. This study found statistically significant differences in VCRs in plants singly infected with different GLRaV-3 variants. Interestingly, no difference in mean VCRs were observed between data sets, despite notable differences in plant age, duration of GLRaV-3 infection, scion/rootstock combination and growing conditions. Several miRNAs showed statistically significant expression modulation between infected and healthy samples. miRNA expression between data sets varied substantially and a greater overall miRNA response was observed in plants with more established GLRaV-3 infections. The lack of significant differences in mean VCRs between data sets, coupled with the consistent modulation of certain miRNAs in plants that have likely been infected for longer is a promising result. This finding could indicate that successful inhibition of further virus replication by plant defence mechanisms occurred, and that these miRNAs are implicated in this response.
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