{"title":"苏云金芽孢杆菌菌株对豌豆芽生长和代谢过程的影响","authors":"A. Kryzhko, N. N. Smagliy","doi":"10.21285/2227-2925-2022-12-4-557-565","DOIUrl":null,"url":null,"abstract":"Although the entomopathogenic bacteria Bacillus thuringiensis Berliner are well-known bio-agents for regulating the population of leaf-eating pests of agricultural and ornamental crops, other agricultural properties of this microorganism are promising. In this work, the growth-stimulating effect of entomopathogenic strains of B. thuringiensis on Deviz pea plants was studied. The entomopathogenic strains B. thuringiensis 685, 926 and 109-C obtained from the Crimean Collection of Microorganisms of the Crimean Agricultural Research Institute, registered online (http://www.ckp-rf.ru) with number 507484, were used as research material. The morphometric parameters of pea sprouts were evaluated following standard methods. Biochemical parameters of pea sprouts were determined for 10-day-old sprouts. The amylase activity of pea sprouts was determined by photocalorimetry; total acidity was measured by titration using 0.1 n NaOH solution; total water-soluble phenolic compounds were determined by Leventhal titrimetric method. It was found that liquid spore culture of strains B. thuringiensis 685, 926 and 109-C had a stimulating effect on the length of root and stem and the weight of 10-day-old sprouts of Deviz pea variety. Treatment with a spore suspension of all tested strains B. thuringiensis led to an increase in the content of organic acids in the sprouts by an average of 12.4% compared with that of the control. Maximum stimulating effect on amylolytic activity and synthesis of phenolic compounds in Deviz pea sprouts was achieved by treating with a spore suspension of the strain B. thuringiensis 926. The amylase activity increased on average by 41.5% when compared to that of the control, while the total content of phenolic compounds in this experiment was 2.3 times higher than that of the control. Therefore, in light of the entomopathogenic properties and the obtained data on the growth-stimulating activity of the strains of B. thuringiensis, it can be concluded that these bacteria have additional potential for their use in agriculture as a bio-agent for plant protection having a complex action.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"7 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Bacillus thuringiensis strains on growth and metabolic processes in Pisum sativum L. sprouts\",\"authors\":\"A. Kryzhko, N. N. Smagliy\",\"doi\":\"10.21285/2227-2925-2022-12-4-557-565\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Although the entomopathogenic bacteria Bacillus thuringiensis Berliner are well-known bio-agents for regulating the population of leaf-eating pests of agricultural and ornamental crops, other agricultural properties of this microorganism are promising. In this work, the growth-stimulating effect of entomopathogenic strains of B. thuringiensis on Deviz pea plants was studied. The entomopathogenic strains B. thuringiensis 685, 926 and 109-C obtained from the Crimean Collection of Microorganisms of the Crimean Agricultural Research Institute, registered online (http://www.ckp-rf.ru) with number 507484, were used as research material. The morphometric parameters of pea sprouts were evaluated following standard methods. Biochemical parameters of pea sprouts were determined for 10-day-old sprouts. The amylase activity of pea sprouts was determined by photocalorimetry; total acidity was measured by titration using 0.1 n NaOH solution; total water-soluble phenolic compounds were determined by Leventhal titrimetric method. It was found that liquid spore culture of strains B. thuringiensis 685, 926 and 109-C had a stimulating effect on the length of root and stem and the weight of 10-day-old sprouts of Deviz pea variety. Treatment with a spore suspension of all tested strains B. thuringiensis led to an increase in the content of organic acids in the sprouts by an average of 12.4% compared with that of the control. Maximum stimulating effect on amylolytic activity and synthesis of phenolic compounds in Deviz pea sprouts was achieved by treating with a spore suspension of the strain B. thuringiensis 926. The amylase activity increased on average by 41.5% when compared to that of the control, while the total content of phenolic compounds in this experiment was 2.3 times higher than that of the control. Therefore, in light of the entomopathogenic properties and the obtained data on the growth-stimulating activity of the strains of B. thuringiensis, it can be concluded that these bacteria have additional potential for their use in agriculture as a bio-agent for plant protection having a complex action.\",\"PeriodicalId\":20601,\"journal\":{\"name\":\"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY\",\"volume\":\"7 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21285/2227-2925-2022-12-4-557-565\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21285/2227-2925-2022-12-4-557-565","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
苏云金芽孢杆菌(Bacillus thuringiensis Berliner)是众所周知的调控农业和观赏作物食叶害虫种群的生物制剂,但这种微生物的其他农业特性也很有前景。本文研究了苏云金芽孢杆菌(B. thuringiensis)昆虫致病菌株对豌豆植株的促生长作用。研究材料为苏云金芽孢杆菌685、926和109-C昆虫病原菌株,该菌株来自克里米亚农业研究所的Crimean Collection of microbiology,其在线注册编号为507484 (http://www.ckp-rf.ru)。采用标准方法对豌豆芽的形态计量学参数进行评价。测定了10 d豌豆芽的生化指标。采用光热法测定豌豆芽淀粉酶活性;用0.1 n NaOH溶液滴定测定总酸度;总水溶性酚类化合物用Leventhal滴定法测定。结果表明,苏云金芽孢杆菌685、926和109-C的液体孢子培养对德维兹豌豆品种的根、茎长和10 d芽重均有促进作用。用所有试验菌株的孢子悬浮液处理后,豆芽中有机酸的含量比对照平均增加了12.4%。苏云金芽孢杆菌926孢子悬浮液对豌豆芽的酶解活性和酚类化合物合成的刺激作用最大。淀粉酶活性比对照平均提高41.5%,酚类化合物总含量比对照提高2.3倍。因此,根据苏云金芽孢杆菌菌株的昆虫病原性和已获得的促生长活性数据,可以得出结论,这些细菌作为具有复杂作用的植物保护生物剂在农业上具有额外的潜力。
Effect of Bacillus thuringiensis strains on growth and metabolic processes in Pisum sativum L. sprouts
Although the entomopathogenic bacteria Bacillus thuringiensis Berliner are well-known bio-agents for regulating the population of leaf-eating pests of agricultural and ornamental crops, other agricultural properties of this microorganism are promising. In this work, the growth-stimulating effect of entomopathogenic strains of B. thuringiensis on Deviz pea plants was studied. The entomopathogenic strains B. thuringiensis 685, 926 and 109-C obtained from the Crimean Collection of Microorganisms of the Crimean Agricultural Research Institute, registered online (http://www.ckp-rf.ru) with number 507484, were used as research material. The morphometric parameters of pea sprouts were evaluated following standard methods. Biochemical parameters of pea sprouts were determined for 10-day-old sprouts. The amylase activity of pea sprouts was determined by photocalorimetry; total acidity was measured by titration using 0.1 n NaOH solution; total water-soluble phenolic compounds were determined by Leventhal titrimetric method. It was found that liquid spore culture of strains B. thuringiensis 685, 926 and 109-C had a stimulating effect on the length of root and stem and the weight of 10-day-old sprouts of Deviz pea variety. Treatment with a spore suspension of all tested strains B. thuringiensis led to an increase in the content of organic acids in the sprouts by an average of 12.4% compared with that of the control. Maximum stimulating effect on amylolytic activity and synthesis of phenolic compounds in Deviz pea sprouts was achieved by treating with a spore suspension of the strain B. thuringiensis 926. The amylase activity increased on average by 41.5% when compared to that of the control, while the total content of phenolic compounds in this experiment was 2.3 times higher than that of the control. Therefore, in light of the entomopathogenic properties and the obtained data on the growth-stimulating activity of the strains of B. thuringiensis, it can be concluded that these bacteria have additional potential for their use in agriculture as a bio-agent for plant protection having a complex action.