{"title":"葫芦巴碱和西格列汀对烟酰胺链脲佐菌素诱导的Wistar大鼠糖尿病的降糖作用","authors":"Hemant V. Kamble, Subhash L. Bodhankar","doi":"10.1016/j.biomag.2013.05.006","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>The objective of the present study was to evaluate the protective effect of trigonelline (TRIG) and </span>sitagliptin<span><span> (SITA) combination in streptozotocin-nicotinamide induced diabetes in Wistar rats. Diabetes was induced by </span>streptozotocin (65</span></span> <!-->mg/kg i.p.) injected 15<!--> <span>min after nicotinamide (110</span> <!-->mg/kg, i.p.). The rats were divided into following groups: Group 1: non-diabetic control, Group 2: diabetic control (saline), Groups 3, 4, 5 received TRIG as 25, 50, 100<!--> <!-->mg/kg p.o., Groups 6, 7, 8 received SITA as 2.5, 5, 10<!--> <!-->mg/kg p.o. respectively. For acute study, serum glucose (SG) levels were measured at 0, 2nd, 4th, 6th and 24th hour after administration. To find out effective combination, acute study of TRIG<!--> <!-->+<!--> <!-->SITA combination was done. The doses used are in combination TRIG<!--> <!-->+<!--> <!-->SITA as Groups 9, 10, 11 as 70%<!--> <!-->+<!--> <!-->30%, 50%<!--> <!-->+<!--> <!-->50%, 30<!--> <!-->+<!--> <!-->70%. SG was measured as per above-mentioned pattern. A 28-day subacute study was performed by taking effective percentage combination of TRIG<!--> <!-->+<!--> <!-->SITA. For subacute study SG, body weight was measured on 7th, 14th, 21st, 28th day; while serum insulin, HbA<sub>1c</sub> and pancreatic histopathological study were done on 28th day. In acute study, TRIG<!--> <!-->+<!--> <!-->SITA (50%<!--> <!-->+<!--> <!-->50%) were more effectively 43.08% reduction in SG level at 6<!--> <!-->h with onset at 2<!--> <!-->h and effect waned at 24<!--> <!-->h. Subacute study reveals that the TRIG<!--> <!-->+<!--> <!-->SITA (50%<!--> <!-->+<!--> <!-->50%) reduces SG by 54.72% than alone on 28th day. Serum insulin levels, body weight, pancreatic mass were increased with reduction in HbA<sub>1c</sub> levels in animals receiving 50%<!--> <!-->+<!--> <!-->50% combination of TRIG<!--> <!-->+<!--> <!-->SITA. The concomitant administration of TRIG<!--> <!-->+<!--> <!-->SITA (50%<!--> <!-->+<!--> <!-->50%) contributes in the prevention to development diabetes and showed synergistic antihyperglycemic effect.</p></div>","PeriodicalId":100181,"journal":{"name":"Biomedicine & Aging Pathology","volume":"3 3","pages":"Pages 125-130"},"PeriodicalIF":0.0000,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biomag.2013.05.006","citationCount":"11","resultStr":"{\"title\":\"Antihyperglycemic activity of trigonelline and sitagliptin in nicotinamide-streptozotocin induced diabetes in Wistar rats\",\"authors\":\"Hemant V. Kamble, Subhash L. Bodhankar\",\"doi\":\"10.1016/j.biomag.2013.05.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span>The objective of the present study was to evaluate the protective effect of trigonelline (TRIG) and </span>sitagliptin<span><span> (SITA) combination in streptozotocin-nicotinamide induced diabetes in Wistar rats. Diabetes was induced by </span>streptozotocin (65</span></span> <!-->mg/kg i.p.) injected 15<!--> <span>min after nicotinamide (110</span> <!-->mg/kg, i.p.). The rats were divided into following groups: Group 1: non-diabetic control, Group 2: diabetic control (saline), Groups 3, 4, 5 received TRIG as 25, 50, 100<!--> <!-->mg/kg p.o., Groups 6, 7, 8 received SITA as 2.5, 5, 10<!--> <!-->mg/kg p.o. respectively. For acute study, serum glucose (SG) levels were measured at 0, 2nd, 4th, 6th and 24th hour after administration. To find out effective combination, acute study of TRIG<!--> <!-->+<!--> <!-->SITA combination was done. The doses used are in combination TRIG<!--> <!-->+<!--> <!-->SITA as Groups 9, 10, 11 as 70%<!--> <!-->+<!--> <!-->30%, 50%<!--> <!-->+<!--> <!-->50%, 30<!--> <!-->+<!--> <!-->70%. SG was measured as per above-mentioned pattern. A 28-day subacute study was performed by taking effective percentage combination of TRIG<!--> <!-->+<!--> <!-->SITA. For subacute study SG, body weight was measured on 7th, 14th, 21st, 28th day; while serum insulin, HbA<sub>1c</sub> and pancreatic histopathological study were done on 28th day. In acute study, TRIG<!--> <!-->+<!--> <!-->SITA (50%<!--> <!-->+<!--> <!-->50%) were more effectively 43.08% reduction in SG level at 6<!--> <!-->h with onset at 2<!--> <!-->h and effect waned at 24<!--> <!-->h. Subacute study reveals that the TRIG<!--> <!-->+<!--> <!-->SITA (50%<!--> <!-->+<!--> <!-->50%) reduces SG by 54.72% than alone on 28th day. Serum insulin levels, body weight, pancreatic mass were increased with reduction in HbA<sub>1c</sub> levels in animals receiving 50%<!--> <!-->+<!--> <!-->50% combination of TRIG<!--> <!-->+<!--> <!-->SITA. The concomitant administration of TRIG<!--> <!-->+<!--> <!-->SITA (50%<!--> <!-->+<!--> <!-->50%) contributes in the prevention to development diabetes and showed synergistic antihyperglycemic effect.</p></div>\",\"PeriodicalId\":100181,\"journal\":{\"name\":\"Biomedicine & Aging Pathology\",\"volume\":\"3 3\",\"pages\":\"Pages 125-130\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.biomag.2013.05.006\",\"citationCount\":\"11\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedicine & Aging Pathology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2210522013000245\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedicine & Aging Pathology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2210522013000245","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Antihyperglycemic activity of trigonelline and sitagliptin in nicotinamide-streptozotocin induced diabetes in Wistar rats
The objective of the present study was to evaluate the protective effect of trigonelline (TRIG) and sitagliptin (SITA) combination in streptozotocin-nicotinamide induced diabetes in Wistar rats. Diabetes was induced by streptozotocin (65 mg/kg i.p.) injected 15 min after nicotinamide (110 mg/kg, i.p.). The rats were divided into following groups: Group 1: non-diabetic control, Group 2: diabetic control (saline), Groups 3, 4, 5 received TRIG as 25, 50, 100 mg/kg p.o., Groups 6, 7, 8 received SITA as 2.5, 5, 10 mg/kg p.o. respectively. For acute study, serum glucose (SG) levels were measured at 0, 2nd, 4th, 6th and 24th hour after administration. To find out effective combination, acute study of TRIG + SITA combination was done. The doses used are in combination TRIG + SITA as Groups 9, 10, 11 as 70% + 30%, 50% + 50%, 30 + 70%. SG was measured as per above-mentioned pattern. A 28-day subacute study was performed by taking effective percentage combination of TRIG + SITA. For subacute study SG, body weight was measured on 7th, 14th, 21st, 28th day; while serum insulin, HbA1c and pancreatic histopathological study were done on 28th day. In acute study, TRIG + SITA (50% + 50%) were more effectively 43.08% reduction in SG level at 6 h with onset at 2 h and effect waned at 24 h. Subacute study reveals that the TRIG + SITA (50% + 50%) reduces SG by 54.72% than alone on 28th day. Serum insulin levels, body weight, pancreatic mass were increased with reduction in HbA1c levels in animals receiving 50% + 50% combination of TRIG + SITA. The concomitant administration of TRIG + SITA (50% + 50%) contributes in the prevention to development diabetes and showed synergistic antihyperglycemic effect.