葫芦巴碱和西格列汀对烟酰胺链脲佐菌素诱导的Wistar大鼠糖尿病的降糖作用

Hemant V. Kamble, Subhash L. Bodhankar
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引用次数: 11

摘要

本研究旨在探讨葫芦巴碱(TRIG)和西格列汀(sitagliptin)联合用药对链脲佐菌素-烟酰胺诱导的Wistar大鼠糖尿病的保护作用。烟酰胺(110 mg/kg, i.p)后15 min注射链脲佐菌素(65 mg/kg, i.p)诱导糖尿病。将大鼠分为3组:1组为非糖尿病对照组,2组为糖尿病对照组(生理盐水),3、4、5组分别给予TRIG 25、50、100 mg/kg p.o., 6、7、8组分别给予SITA 2.5、5、10 mg/kg p.o.。急性组于给药后0、2、4、6、24小时测定血清葡萄糖(SG)水平。为寻找有效的联合用药,对TRIG + SITA联合用药进行了急性研究。使用的剂量是TRIG + SITA组合作为第9、10、11组,分别为70% + 30%、50% + 50%、30 + 70%。SG按上述模式测定。通过TRIG + SITA的有效百分比组合进行了为期28天的亚急性研究。亚急性期SG于第7、14、21、28天测定体重;第28天进行血清胰岛素、糖化血红蛋白及胰腺组织病理学检查。在急性期研究中,TRIG + SITA(50% + 50%)更有效地在6小时降低SG水平43.08%,在2小时起效,在24小时效果减弱。亚急性期研究显示,TRIG + SITA(50% + 50%)在第28天比单独使用更有效地降低SG水平54.72%。在接受50% + 50% TRIG + SITA联合治疗的动物中,血清胰岛素水平、体重、胰腺质量随着HbA1c水平的降低而增加。同时给予TRIG + SITA(50% + 50%)有助于预防糖尿病的发生,并显示出协同降糖作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antihyperglycemic activity of trigonelline and sitagliptin in nicotinamide-streptozotocin induced diabetes in Wistar rats

The objective of the present study was to evaluate the protective effect of trigonelline (TRIG) and sitagliptin (SITA) combination in streptozotocin-nicotinamide induced diabetes in Wistar rats. Diabetes was induced by streptozotocin (65 mg/kg i.p.) injected 15 min after nicotinamide (110 mg/kg, i.p.). The rats were divided into following groups: Group 1: non-diabetic control, Group 2: diabetic control (saline), Groups 3, 4, 5 received TRIG as 25, 50, 100 mg/kg p.o., Groups 6, 7, 8 received SITA as 2.5, 5, 10 mg/kg p.o. respectively. For acute study, serum glucose (SG) levels were measured at 0, 2nd, 4th, 6th and 24th hour after administration. To find out effective combination, acute study of TRIG + SITA combination was done. The doses used are in combination TRIG + SITA as Groups 9, 10, 11 as 70% + 30%, 50% + 50%, 30 + 70%. SG was measured as per above-mentioned pattern. A 28-day subacute study was performed by taking effective percentage combination of TRIG + SITA. For subacute study SG, body weight was measured on 7th, 14th, 21st, 28th day; while serum insulin, HbA1c and pancreatic histopathological study were done on 28th day. In acute study, TRIG + SITA (50% + 50%) were more effectively 43.08% reduction in SG level at 6 h with onset at 2 h and effect waned at 24 h. Subacute study reveals that the TRIG + SITA (50% + 50%) reduces SG by 54.72% than alone on 28th day. Serum insulin levels, body weight, pancreatic mass were increased with reduction in HbA1c levels in animals receiving 50% + 50% combination of TRIG + SITA. The concomitant administration of TRIG + SITA (50% + 50%) contributes in the prevention to development diabetes and showed synergistic antihyperglycemic effect.

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