重要药用植物扁豆的组织培养优化

IF 0.4 4区 生物学 Q4 Agricultural and Biological Sciences
T. Mahmood, H. Arshad, W. Akhtar, M. Ilyas, Tariq Habib, Z. Shinwari
{"title":"重要药用植物扁豆的组织培养优化","authors":"T. Mahmood, H. Arshad, W. Akhtar, M. Ilyas, Tariq Habib, Z. Shinwari","doi":"10.21826/2446-82312021V76E2021009","DOIUrl":null,"url":null,"abstract":"This study was designed to develop an efficient protocol for Lallementia royleana in vitro culturing and to analyze the effect of various hormones on its culturing ability. The indirect organogenesis and its callogenetic ability were evaluated; with the best (100%) results with 1 mg L-1 of benzyl aminopurine (BAP) and 2 mg L-1 of indole acetic acid (IAA) combination. In direct regeneration, leave petioles, nodes and internodes explants were cultured for shooting on various concentrations of BAP and kinetin (KIN). Maximum shooting (80%) was obtained from nodal parts with 1.0 mg L-1 of BAP and 0.5 mg L-1 of KIN. Similarly, the best in vitro rooting (24 roots) was achieved with 1 mg L-1 naphthalene acetic acid (NAA). Lallementia royleana showed higher regeneration through direct regeneration than callogenesis. Significant variability (p = 0.002625) among callus induction, plant parts and hormonal combinations were observed. The node and internode explants showed observable callus responses in combinations of 2, 4-Dichlorophenoxyacetic acid (2,4-D) with NAA, and BAP with IAA.","PeriodicalId":54998,"journal":{"name":"Iheringia Serie Botanica","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Tissue culture optimization for Lallementia royleana L. an important medicinal plant\",\"authors\":\"T. Mahmood, H. Arshad, W. Akhtar, M. Ilyas, Tariq Habib, Z. Shinwari\",\"doi\":\"10.21826/2446-82312021V76E2021009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study was designed to develop an efficient protocol for Lallementia royleana in vitro culturing and to analyze the effect of various hormones on its culturing ability. The indirect organogenesis and its callogenetic ability were evaluated; with the best (100%) results with 1 mg L-1 of benzyl aminopurine (BAP) and 2 mg L-1 of indole acetic acid (IAA) combination. In direct regeneration, leave petioles, nodes and internodes explants were cultured for shooting on various concentrations of BAP and kinetin (KIN). Maximum shooting (80%) was obtained from nodal parts with 1.0 mg L-1 of BAP and 0.5 mg L-1 of KIN. Similarly, the best in vitro rooting (24 roots) was achieved with 1 mg L-1 naphthalene acetic acid (NAA). Lallementia royleana showed higher regeneration through direct regeneration than callogenesis. Significant variability (p = 0.002625) among callus induction, plant parts and hormonal combinations were observed. The node and internode explants showed observable callus responses in combinations of 2, 4-Dichlorophenoxyacetic acid (2,4-D) with NAA, and BAP with IAA.\",\"PeriodicalId\":54998,\"journal\":{\"name\":\"Iheringia Serie Botanica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2021-05-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iheringia Serie Botanica\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.21826/2446-82312021V76E2021009\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iheringia Serie Botanica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.21826/2446-82312021V76E2021009","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

摘要

本研究旨在建立一种高效的罗氏小扁豆离体培养方案,并分析各种激素对其培养能力的影响。评价了间接器官发生及其胼胝质形成能力;以1 mg L-1的苄基氨基嘌呤(BAP)和2 mg L-1的吲哚乙酸(IAA)组合为最佳(100%)。在直接再生中,叶片叶柄、节和节间外植体在不同浓度的BAP和动素(KIN)处理下进行拔节培养。当BAP 1.0 mg L-1, KIN 0.5 mg L-1时,茎节部位的发芽率最高(80%)。同样,1 mg L-1萘乙酸(NAA)的离体生根效果最好(24根)。小扁豆直接再生比胼胝质形成具有更高的再生能力。愈伤组织诱导、植株部位和激素组合之间存在显著差异(p = 0.002625)。2,4-二氯苯氧乙酸(2,4- d)与NAA、BAP与IAA组合处理后,茎节和节间外植体均表现出明显的愈伤组织反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tissue culture optimization for Lallementia royleana L. an important medicinal plant
This study was designed to develop an efficient protocol for Lallementia royleana in vitro culturing and to analyze the effect of various hormones on its culturing ability. The indirect organogenesis and its callogenetic ability were evaluated; with the best (100%) results with 1 mg L-1 of benzyl aminopurine (BAP) and 2 mg L-1 of indole acetic acid (IAA) combination. In direct regeneration, leave petioles, nodes and internodes explants were cultured for shooting on various concentrations of BAP and kinetin (KIN). Maximum shooting (80%) was obtained from nodal parts with 1.0 mg L-1 of BAP and 0.5 mg L-1 of KIN. Similarly, the best in vitro rooting (24 roots) was achieved with 1 mg L-1 naphthalene acetic acid (NAA). Lallementia royleana showed higher regeneration through direct regeneration than callogenesis. Significant variability (p = 0.002625) among callus induction, plant parts and hormonal combinations were observed. The node and internode explants showed observable callus responses in combinations of 2, 4-Dichlorophenoxyacetic acid (2,4-D) with NAA, and BAP with IAA.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Iheringia Serie Botanica
Iheringia Serie Botanica PLANT SCIENCES-
CiteScore
0.70
自引率
0.00%
发文量
22
审稿时长
>12 weeks
期刊介绍: The Iheringia, Série Botânica is a publication edited by the Museu de Ciências Naturais, Fundação Zoobotânica do Rio Grande do Sul, since 1958. The name Iheringia is actually a tribute to Hermann von Ihering (a German researcher who became a Brazilian citizen) and his important studies in the Natural Sciences.The journal has the mission of publishing articles, reviews and original scientific notes that cover diverse areas in Botany, and may be written in Portuguese, Spanish and English. The journal is widely available in both international and national scientific community. The issues are published and distributed twice a year to 118 national institutions and 152 international institutions. The distribution is either free of charge or by exchange. Impac Factor JCR 2014/2015 = 0.263 Qualis CAPES 2015: B 4 (Biodiversity) B1 (Agricultural Science I) B1 (Environment)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信