The evaluation of environmental waters using yeast DNA microarray

We propose a new method for the evaluation of environmental water by using DNA microarray technology. Twenty-one types of environmental waters were sampled from incineration processes, some factories, and various soils. We performed a comprehensive analysis by DNA microarray, and attempted to classify the environmental water samples by hierarchical cluster analysis. These water samples were classified into clusters from A to F together with chemicals and physical stress conditions. The water sample that grouped into cluster A caused protein denaturation and oxidative stress, but not mutagenesis. The water samples that grouped into cluster B caused protein denaturation. The water samples that grouped into cluster C caused mutagenesis, protein denaturation, and cell wall damage. The water samples that grouped into cluster D caused mutagenesis. The water samples that grouped into cluster E caused protein denaturation and oxidative stress. The water samples that grouped into cluster F caused oxidative stress. Consequently, the potential influences of environmental water could be estimated by hierarchical cluster analysis.