V. Tsvetkov, L. Yarullina, A. Sorokan, V. Khabibullina, I. Mardanshin
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引用次数: 0
摘要
研究了枯草芽孢杆菌与水杨酸(SA)和茉莉酸(JA)联合施用对马铃薯叶片淀粉酶、纤维素酶、蛋白酶及其抑制剂活性的影响,并对马铃薯在缺水条件下对疫霉(Mont.) de Bary抗性的形成进行了研究。用枯草芽孢杆菌悬浮液(108个细胞/mL)、SA(10−6 M)、JA(10−7 M)和SA + JA的混合菌液处理微块茎生长的植株,然后用病原菌感染(107个孢子/mL),在干旱条件下培养。枯草芽孢杆菌处理,特别是与信号分子结合,有助于减少在缺乏水分的情况下生长的植物的病原体侵染程度。水杨酸盐和茉莉酸盐信号通路都在水解酶活性调控和植物抗性刺激中发挥重要作用。在枯草芽孢杆菌和信号分子的影响下,水解酶抑制剂的激活程度存在差异,这表明干旱条件下马铃薯对病原菌的抗性形成途径不同。
Activity of Hydrolases and Their Inhibitors in Potato Plants Treated with Bacillus subtilis, Salicylic, and Jasmonic Acids and Affected by the Combined Effect of the Late Blight and the Lack of Moisture
The effect of Bacillus subtilis in combination with salicylic (SA) and jasmonic (JA) acids on the activity of amylases, cellulases, proteases, and their inhibitors in potato leaves in connection with the development of resistance to Phytophthora infestans (Mont.) de Bary in conditions of moisture deficiency have been investigated. Plants grown from microtubers were treated with Bacillus subtilis suspension (108 cells/mL) and with a mixture of bacteria with SA (10−6 M), JA (10−7 M), and SA + JA and were then infected with P. infestans (107 spores/mL) and cultivated under drought. Treatment with B. subtilis bacteria, especially in combination with signaling molecules, contributed to a decrease in the degree of pathogen infestation on plants grown with a lack of moisture. Both salicylate and jasmonate signaling pathways play an important role in the regulation of hydrolase activity and the stimulation of plant resistance. The revealed differences in the degree of hydrolase inhibitors activation under the influence of B. subtilis bacteria and signal molecules suggest different paths to the formation of resistance to P. infestans in potato under drought conditions.
期刊介绍:
The International Journal of Plant Biology is an Open Access, online-only, peer-reviewed journal that considers scientific papers in all different subdisciplines of plant biology, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, mycology and phytopathology.