国产牛奶品质检测碱性磷酸酶试剂盒的研制

S. Mazhar, Naaz Abbas, Y. Saleem, Q. Syed, S. Riaz, Ramsha Essa, Bakhtawar Bukhari, Saira Ashfaq, Ishrat Perveen, Syed Hussain Imam Abidi
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引用次数: 0

摘要

牛奶是一种营养丰富的食品,为大量微生物的生长发育提供了良好的环境和营养。微生物质量保证技术通常可以作为一种快速的策略来调查粗奶和巴氏杀菌奶的微生物性质。目的:研制国产牛奶质量、微生物存在、巴氏灭菌乳和非巴氏灭菌乳的快速检测和鉴别试剂盒。方法:从拉合尔不同地理区域和不同品牌的巴氏奶中采集14份生奶和巴氏奶样品。制备了原生碱性磷酸酶乳质量比色检测试剂盒,并研制了200个反应的比色检测试剂盒。对碱性磷酸酶试剂盒在不同温度和牛奶体积下进行了检测。结果:结果表明,从当地商店和附近市场购买的各种牛奶价格过高,牛奶质量在微生物存在方面没有差异。此外,巴氏奶的不同效果,经确认试验后品种保持蓝色,没有变化。结论:本试剂盒是一种快速的检测方法,可用于基于微生物存在的牛奶质量鉴定,因此,巴氏灭菌或未经巴氏灭菌的牛奶也可在现场进行检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of Indigenous Alkaline Phosphatase Kit for the Detection of Milk Quality
Milk is a profoundly nutritious food that provides the favorable environment and nutrition for the growth development of large number of microorganisms. Microbiological quality assurance techniques could be usually utilized as a speedy strategy to survey the microbiological nature of crude and pasteurized milk. Objective: To develop indigenous rapid kit for determination and differentiation of milk quality, microbial presence, pasteurized and unpasteurized milk. Methods: Some 14-milk raw and pasteurized milk samples were collected from different geographical areas of Lahore and different brands of pasteurized milk. The colorimetric indigenous alkaline phosphatase milk quality detection kit was prepared for 200 reactions was developed. Alkaline phosphatase kit was tested at different temperature and volume of milk. Results: Results showed that a wide range of milk that bought from local stores and nearby market with exorbitant cost milk types shown no difference in milk quality in terms of presence of microbes. Moreover, different effect of pasteurized milk was observed after affirm test the variety stayed blue and not changed. Conclusions: This indigenous kit is test is quick monetary strategy that can be utilized for identification of milk quality on the basis of microbial presence, therefore, pasteurized or unpasteurized milk can be tested in field as well.
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