鲶鱼(Clarias sp.)蛋白水解物作为微生物参考材料候选基质的效价研究

Q3 Environmental Science
E. Kurniawati, B. Ibrahim, Desniar Desniar
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引用次数: 2

摘要

鱼蛋白水解物(FPH)是一种含有较小肽和氨基酸的鱼蛋白衍生物。FPH产品水溶性高,乳化能力好,膨胀能力大。由于其功能特性,它允许FPH用作制造二级微生物标准物质的原料。本研究旨在对鲶鱼(Clarias sp.) FPH作为微生物二级参比基质的候选物进行表征。通过酶解、冷冻干燥、磨粉制备FPH。用5% (w/w)的木瓜蛋白酶,55℃水解5小时,80℃加热20分钟停止木瓜蛋白酶活性。将FPH与明胶、谷氨酸钠、葡萄糖溶液混合,加入肠炎沙门氏菌(sv)冷冻干燥。结果表明,鲶鱼FPH为黄白色粉末,FPH产率为11.05%。FPH含量为3.77±0.12%,灰分含量为7.26±0.03%,蛋白质含量为86.09±0.17%,脂肪含量为1.38±0.07%。蛋白含量高于脱脂奶(33.42%)。鲶鱼FPH和脱脂奶的碳水化合物含量分别为1.56%和57.46%。在沙门氏菌最高活力和均质性条件下,鲶鱼FPH的最佳浓度为14%。候选标准物质在-20℃的储存温度下是稳定的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Potency of Catfish (Clarias sp.) Protein Hydrolysates as Candidates Matrices for Microbiology Reference Material
Fish protein hydrolysate (FPH) is a derivative product of fish proteins containing smaller peptides and amino acids. FPH products have high water solubility, good emulsion capacity, and large expanding ability. With its functional properties, it allows FPH to be used as a raw material in the manufacturing of secondary microbiological reference materials. This study was intended to characterize catfish (Clarias sp.) FPH as a candidate for the matrix of microbial secondary reference. The FPH was prepared through enzymatic hydrolysis, freeze-drying and milling. The hydrolysis processes were carried out using 5% (w/w) papain, 55 °C for 5 hours, then the papain activity was stopped by increasing the temperature to 80 °C for 20 minutes.The FPH was combined with gelatine, sodium glutamate, glucose solution, and was spiked with Salmonella enteritica sv Enteritidis and freeze-dried. Results showed that catfish FPH was yellowish-white powder with a FPH yield of 11.05%. The proximate analysis of FPH revealed the moisture content of 3.77 ± 0.12%, ash content of 7.26 ± 0.03%, protein content of 86.09 ± 0.17%, and fat content of 1.38 ± 0.07%. The protein content of the FPH was greater than skim milk (33.42%). Carbohydrate levels of catfish FPH and skim milk were 1.56% and 57.46%, respectively. The best concentration of catfish FPH to perform as a microbiological reference material was 14%, obtained from highest viability of Salmonella bacteria and homogeny. The candidate for reference material were stable at storage temperatures of -20 oC.
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
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