气道成纤维细胞分泌产物促进细胞迁移

IF 0.5 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Current Proteomics Pub Date : 2021-08-23 DOI:10.2174/1570164618666210823094105

Nundisa Jaulin, R. Idrus, A. Saim, W. I. Wan-Ibrahim, P. S. Abdul-Rahman, Y. Lokanathan

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引用次数: 1

摘要

鼻腔成纤维细胞分泌组，包括各种细胞因子、趋化因子和生长因子，促进细胞迁移。目前，气道成纤维细胞(AF)条件培养基(AFCM)的蛋白质组学研究正处于积极阶段。本研究旨在分析和鉴定AF分泌的蛋白，这些蛋白可以促进气道上皮的伤口愈合，并预测其潜在的通路。从多余的人鼻甲中分离气道上皮细胞(AECs)和af细胞并进行培养。采用无血清气道上皮基础培养基(AECM)或无血清F12:DMEM (FDCM)培养afm收集AFCM。为了评估细胞迁移，AECs补充气道上皮培养基和定义角化细胞培养基(1:1;AEDK;对照)，或AEDK中添加20% AECM或20% FDCM。质谱样品采用蛋白沉淀、凝胶电泳和凝胶内消化制备。与FDCM和对照培养基相比，AECM促进了更好的细胞迁移。生物信息学分析鉴定出AECM和FDCM中分别有121和92个蛋白，其中109和82个为分泌蛋白。STRING®分析预测，来自AECM的23个蛋白和来自FDCM的16个蛋白参与伤口愈合。条件培养基通过增强细胞迁移来促进伤口愈合，我们成功地鉴定了条件培养基中多种在伤口愈合中起重要作用的分泌蛋白。

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Airway Fibroblast Secretory Products Enhance Cell Migration

The nasal fibroblast secretome, which includes various cytokines, chemokines, and growth factors, promotes cell migration. Currently, the proteomics of airway fibroblast (AF) conditioned medium (AFCM) are being actively studied. This study was aimed at profiling and identifying the AF secreted proteins that can enhance wound healing of the airway epithelium and predict the potential pathway involved. Airway epithelial cells (AECs) and AFs were isolated from redundant human nasal turbinate and cultured. AFCM was collected by culturing the AFs either with serum-free airway epithelium basal medium (AECM) or with serum-free F12:DMEM (FDCM). For evaluating cell migration, the AECs were supplemented with airway epithelium medium and defined keratinocyte medium (1:1; AEDK; control), or with AEDK supplemented with 20% AECM or 20% FDCM. The mass spectrometry sample was prepared by protein precipitation, followed by gel electrophoresis and in-gel digestion. AECM promoted better cell migration compared to the FDCM and the control medium. Bioinformatics analysis identified a total of 121, and 92 proteins from AECM and FDCM, respectively: 109 and 82 were identified as secreted proteins, respectively. STRING® analysis predicted that 23 proteins from the AECM and 16 proteins from the FDCM are involved in wound healing. Conditioned medium promotes wound healing by enhancing cell migration, and we successfully identified various secretory proteins in a conditioned medium that play important roles in wound healing.

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来源期刊

Current Proteomics BIOCHEMICAL RESEARCH METHODS-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>0 weeks

期刊介绍： Research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed in-depth/mini review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry. Current Proteomics publishes in-depth/mini review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, software, databases, technological advances and applications of proteomics, including functional proteomics. Diverse technologies covered include but are not limited to: Protein separation and characterization techniques 2-D gel electrophoresis and image analysis Techniques for protein expression profiling including mass spectrometry-based methods and algorithms for correlative database searching Determination of co-translational and post- translational modification of proteins Protein/peptide microarrays Biomolecular interaction analysis Analysis of protein complexes Yeast two-hybrid projects Protein-protein interaction (protein interactome) pathways and cell signaling networks Systems biology Proteome informatics (bioinformatics) Knowledge integration and management tools High-throughput protein structural studies (using mass spectrometry, nuclear magnetic resonance and X-ray crystallography) High-throughput computational methods for protein 3-D structure as well as function determination Robotics, nanotechnology, and microfluidics.