{"title":"韩国玉米食品中伏马菌素B1的调查","authors":"Eun-kyung Kim, D. Shon, S. Chung, Young-bae Kim","doi":"10.1080/02652030110113735","DOIUrl":null,"url":null,"abstract":"Seventy-six corn-based foods collected in Seoul, Korea, including corn flakes, corn snack, cornstarch, corn for popping, roasted corn for tea, canned sweet corn and other corn products were analysed for the occurrence of fumonisin B1 (FB1) by using direct competitive enzyme-linked immunosorbent assay (dcELISA) and high-performance liquid chromatography (HPLC). The average recoveries of FB1 from the corn flakes sample in the range 5–1000 ng g−1 were 104% by dcELISA and 82% by HPLC. The limits of detection were approximately 5 ng g—1 by dcELISA and 20 ng g−1 by HPLC. The incidences and mean levels of FB1 were 73.3, 78.6, 50, 58.3, 17.6 and 40% and 41.8, 67.9, 114, 256, 172 and 22 ng g−1 from corn flakes, corn snack, corn starch, corn for popping, roasted corn for tea and other corn products, respectively, by dcELISA. No FB1 was found in canned sweet corn. The results obtained by dcELISA were correlated to those by HPLC for FB1 (r2 = 0.992). This is the first report on the occurrence of FB1 in corn-based foods in Korea.","PeriodicalId":12310,"journal":{"name":"Food Additives & Contaminants","volume":"17 1","pages":"459 - 464"},"PeriodicalIF":0.0000,"publicationDate":"2002-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"17","resultStr":"{\"title\":\"Survey for fumonisin B1 in Korean corn-based food products\",\"authors\":\"Eun-kyung Kim, D. Shon, S. Chung, Young-bae Kim\",\"doi\":\"10.1080/02652030110113735\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Seventy-six corn-based foods collected in Seoul, Korea, including corn flakes, corn snack, cornstarch, corn for popping, roasted corn for tea, canned sweet corn and other corn products were analysed for the occurrence of fumonisin B1 (FB1) by using direct competitive enzyme-linked immunosorbent assay (dcELISA) and high-performance liquid chromatography (HPLC). The average recoveries of FB1 from the corn flakes sample in the range 5–1000 ng g−1 were 104% by dcELISA and 82% by HPLC. The limits of detection were approximately 5 ng g—1 by dcELISA and 20 ng g−1 by HPLC. The incidences and mean levels of FB1 were 73.3, 78.6, 50, 58.3, 17.6 and 40% and 41.8, 67.9, 114, 256, 172 and 22 ng g−1 from corn flakes, corn snack, corn starch, corn for popping, roasted corn for tea and other corn products, respectively, by dcELISA. No FB1 was found in canned sweet corn. The results obtained by dcELISA were correlated to those by HPLC for FB1 (r2 = 0.992). This is the first report on the occurrence of FB1 in corn-based foods in Korea.\",\"PeriodicalId\":12310,\"journal\":{\"name\":\"Food Additives & Contaminants\",\"volume\":\"17 1\",\"pages\":\"459 - 464\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"17\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Additives & Contaminants\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/02652030110113735\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Additives & Contaminants","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/02652030110113735","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 17
摘要
采用直接竞争酶联免疫吸附法(dcELISA)和高效液相色谱法(HPLC)对韩国首尔地区采集的玉米片、玉米零食、玉米淀粉、炸玉米、烘茶玉米、甜玉米罐头等76种玉米制品中伏马菌素B1 (FB1)的含量进行了分析。玉米片样品中FB1在5 ~ 1000 ng g−1范围内的平均回收率分别为104%和82%。dcELISA检出限约为5 ng g - 1, HPLC检出限约为20 ng g - 1。dcELISA检测结果显示，玉米片、玉米零食、玉米淀粉、炸玉米、烘茶玉米和其他玉米制品中FB1的含量分别为73.3、78.6、50、58.3、17.6和40%，平均含量分别为41.8、67.9、114、256、172和22 ng g−1。甜玉米罐头中未发现FB1。dcELISA法与HPLC法测定FB1的结果具有较好的相关性(r2 = 0.992)。这是国内首次报道在玉米食品中发现FB1。
Survey for fumonisin B1 in Korean corn-based food products
Seventy-six corn-based foods collected in Seoul, Korea, including corn flakes, corn snack, cornstarch, corn for popping, roasted corn for tea, canned sweet corn and other corn products were analysed for the occurrence of fumonisin B1 (FB1) by using direct competitive enzyme-linked immunosorbent assay (dcELISA) and high-performance liquid chromatography (HPLC). The average recoveries of FB1 from the corn flakes sample in the range 5–1000 ng g−1 were 104% by dcELISA and 82% by HPLC. The limits of detection were approximately 5 ng g—1 by dcELISA and 20 ng g−1 by HPLC. The incidences and mean levels of FB1 were 73.3, 78.6, 50, 58.3, 17.6 and 40% and 41.8, 67.9, 114, 256, 172 and 22 ng g−1 from corn flakes, corn snack, corn starch, corn for popping, roasted corn for tea and other corn products, respectively, by dcELISA. No FB1 was found in canned sweet corn. The results obtained by dcELISA were correlated to those by HPLC for FB1 (r2 = 0.992). This is the first report on the occurrence of FB1 in corn-based foods in Korea.
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