Modification of bone repair hydroxyapatite scaffold by hydrothermal method with assistance of copperions: Testing the angiogenesis using Chick Chorioallantoic Membrane as ex vivo model

The angiogenesis in the surface of hydroxyapatite scaffolds materials for bone repair was studied by establishing Chick Chorioallantoic Membrane model. Chick Chorioallantoic Membrane model with heavy vascularization was prepared to explore the angiogenesis formations. Hydroxyapatite scaffold has been coated hydrothermally with assistance of copper ions to improve its properties. The morphology of the new product was changed to the flower-like shape (micro/nano-structure) compared with uncoated scaffold. The optical camera, optical microscopic and scanning electron microscope were used to charcterized the investigated results. The investigated results implied that the modified (coated) scaffold can promoted angiogenesis formations compared with the uncoated scaffold. Furthermore, the present tests with chicken chorioallantoic membrane model allow a convenient, efficient, and low-cost method of exploring the angiogenesis on scaffolds surfaces.