霍夫曼原心菌无毒株与有毒株生长及毒性反应的比较

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2020-03-12 DOI:10.3354/ab00725

Thomas Chun-Hung Lee, P. Chan, Steven Jing-Liang Xu, Fred Wang-Fat Lee

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引用次数: 4

摘要

本实验旨在研究无毒菌株(CCMP683)和有毒菌株(CCMP2804)在不同硝酸盐和磷酸盐浓度下的生长和毒性反应。这2株菌株分别在标准、贫或10倍补充硝酸盐或磷酸盐的L1-Si培养基中培养。CCMP683在标准L1-Si培养基中生长迟缓。硝酸盐或磷酸盐的消耗降低了两种菌株的细胞密度。硝酸盐的补充使两个菌株的细胞密度略有增加。磷酸盐的补充也轻微增加了CCMP2804的细胞密度，但令人惊讶的是降低了CCMP683的细胞密度。在CCMP683中未检测到冈田酸及其衍生物。仅在CCMP2804中检测到OA。硝酸盐或磷酸盐的消耗增加了OA的细胞浓度，而硝酸盐或磷酸盐的补充对OA的细胞浓度没有影响。相关分析表明，OA细胞浓度与细胞密度呈负相关。在对磷酸盐补充的生长反应和产生OA的能力方面的差异表明，这两个菌株可能是与磷酸盐代谢和OA毒性相关的比较研究的良好候选者。

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Comparison of growth and toxicity responses between non-toxic and toxic strains of Prorocentrum hoffmannianum

We aimed to study the growth and toxicity responses of non-toxic (CCMP683) and toxic (CCMP2804) strains of Prorocentrum hoffmannianum under various nitrate and phosphate concentrations. The 2 strains were cultured in L1-Si medium with standard, depleted or 10-fold repleted nitrate or phosphate. CCMP683 cultured in standard L1-Si medium exhibited delayed growth. Nitrate or phosphate depletion decreased the cell density of both strains. Repletion of nitrate slightly increased the cell density of both strains. Repletion of phosphate also slightly increased the cell density of CCMP2804 but surprisingly decreased the cell density of CCMP683. Okadaic acid (OA) and its derivatives were not detected in CCMP683. OA was detected only in CCMP2804. Depletion of nitrate or phosphate increased the cellular concentration of OA, and repletion of nitrate or phosphate had no effect on the cellular concentration of OA. Correlation analysis indicated that the cellular concentration of OA was negatively correlated with cell density. Differences in the growth response to phosphate repletion and in the ability to produce OA suggest that the 2 strains may be good candidates for comparative studies related to phosphate metabolism and OA toxicity.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.