戊二醛/THNM复合剂在4个常规油田的微生物防治效果评价

M. Paschoalino, Jon B Raymond, E. Sianawati, Veronica Silva
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引用次数: 0

摘要

在实验室试验中,利用巴西和阿根廷26个油田的水样,对戊二醛和THNM(三(羟甲基)硝基甲烷)的新型协同杀菌剂组合的性能进行了广泛评估。该性能最终在四个现场试验中得到验证,每个国家两个(A1, A2, B1, B2),持续时间超过三个月。在实验室测试中,从各个油田的许多地点收集水样,并通过系列稀释(SRB和APB虫瓶)、ATP和分子生物学技术(NGS)进行表征/枚举。以污染最严重地区的水和分离的本地SRB/APB作为基质和试验接种物,进行了杀菌剂优化试验。通过评估在热老化条件下(60°C)快速杀死原生生物(2小时接触时间)和在较长时间内(≥7天)保护水不受污染的能力,对总有效成分浓度在111至250 ppm之间的许多杀菌剂系统进行了评估。结果表明,戊二醛/THNM在大多数评估样品中提供了最佳性能,因此在现场测试中被选中进行性能评估,因为这种特殊处理在实验室中的性能得到了提高。现场试验采用最低的杀菌剂总浓度,在各种有问题的现场位置(在实验室研究中)显示微生物减少≥4 log10。所有杀菌剂每周分批处理2-3次(每次处理2-3小时)。具体来说,共给药的戊二醛/THNM组合取代了现有的THPS或戊二醛(分批给药)与季铵化合物(连续注射)的联合处理。现场试验B1 -结果显示,在最远的注射井(距离杀菌剂施用点12公里),细菌数量显著减少。处理1个月后,总厌氧菌水平由~106 CFU/mL降至102 CFU/mL以下。此外,与传统使用的现有杀菌剂相比,杀菌剂的总消费量减少了24%。现场试验B2 -在注入水处理后,SRB结果显示,在最远的注入井(30公里),在处理3个月后,从103个细胞/mL降至101个细胞/mL。现场试验A1 -将戊二醛/THNM应用于生产水和注入水中,在处理1个月后,最远的注入井(7公里)SRB/APB水平降低(约108 CFU/mL至102 CFU/mL)。现场试验A2 -在对生产水和注入水进行处理后,所有监测点在6周后均显示SRB计数从~107 CFU/mL降至102-103 CFU/mL。此外,在B1和A1试验中,NGS结果表明微生物种群向危害较小(与mic无关)的微生物转移。总的来说,与传统的化学杀菌剂相比,这种杀菌剂组合的新颖之处在于它能够提供强大的、广谱的抗菌性能和长期的有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of a Glutaraldehyde/THNM Combination for Microbial Control in Four Conventional Oilfields
The performance of a new synergistic biocide combination based on glutaraldehyde and THNM (tris (hydroxymethyl) nitromethane) was extensively evaluated in laboratory trials using water samples from twenty-six Brazilian and Argentinian oilfields. The performance was ultimately validated in four field trials, two per country (A1, A2, B1, B2), over a three month duration. For laboratory tests, water samples were collected from numerous locations of the various oilfields and characterized/enumerated by serial dilution (SRB and APB bug bottles), ATP, and molecular biology techniques (NGS). Water and isolated indigenous SRB/APB from the most contaminated locations were used as the matrix and test inoculum for the biocide optimization tests. Numerous biocide systems, at total active ingredient concentrations ranging from 111 to 250 ppm, were evaluated by assessing the ability to rapidly kill the native organisms (2 hour contact time at room temperature) and protect the water from contamination over a prolonged time frame (≥7 days) under heat-aged conditions (60°C). Results demonstrated that glutaraldehyde/THNM provided the best performance in the majority of the samples evaluated and was therefore selected for performance evaluations in field tests owing to the enhanced performance of this particular treatment in the laboratory. Field trials were conducted by applying the lowest total biocide concentration that demonstrated a ≥ 4 log10 microbial reduction (in the laboratory studies) at various problematic field locations. All biocides were dosed as batch treatments 2-3 times per week (2-3 hours per treatment). Specifically, the co-dosed glutaraldehyde/THNM combination replaced incumbent treatments of either THPS or glutaraldehyde (batch dosed) in combination with a quaternary ammonium compound which was being applied by continuous injection: Field trial B1 – Results showed a significant reduction in bacterial counts at the farthest injection well (12 km from the point of biocide application). Total anaerobic bacteria levels were reduced from ~106 CFU/mL to less than 102 CFU/mL after 1 month treatment. Additionally, total biocide consumption was reduced by 24% as compared to the incumbent biocides traditionally applied. Field trial B2 – Following treatment of injection water, SRB results showed a reduction at the farthest injection well (30 km), from 103 cells/mL to 101 cells/mL, after 3 months treatment. Field trial A1 – After applying glutaraldehyde/THNM to production and injection water, SRB/APB levels were reduced (~108 CFU/mL to 102 CFU/mL) at the farthest injection well (7 km) after 1 month treatment. Field trial A2 – Following the treatment of production and injection water, all monitored points demonstrated a reduction of SRB counts from ~107 CFU/mL to 102-103 CFU/mL after 6 weeks. Furthermore, in the B1 and A1 trials, NGS results indicated a shift of the microbial population to less harmful (non-MIC relevant) organisms. Overall, the novelty of this biocide combination is its ability to provide strong, broad-spectrum antimicrobial performance and long-term effectiveness, as compared to traditional biocide chemistries.
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