番茄耐盐基因型的体外筛选

R. Rashed, M. Roy, S. K. Paul, M. Haque
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引用次数: 10

摘要

土壤盐碱化是制约作物生产的重要非生物胁迫之一。番茄(Solanum lycopersicum L.)具有中等的耐盐性,通常种植在土壤盐碱化的地区。研究了14个番茄基因型在盐胁迫条件下的表型反应。BD-7260植株根长最短,为250 mM,而BD-7302植株根长较长,为11.6 cm。培养基中NaCl浓度对番茄根长和株重影响显著。重量LS均值的基因型分布显示,基因型BARI-2和系BD-7292表现最好,系BD-7762表现最差。这些发现为今后的杂交研究提供了一些有前景的耐盐番茄基因型。分析在不同非生物胁迫下对其他作物表现出保护作用的新基因以及一些已鉴定的基因,如PIPs、LTPs、AGPs、PRPs、GRPs等,是非常必要的,这些基因在这些筛选的番茄基因型中的存在和表达模式,将为这些基因在转基因植物中的过表达提供有力的信息,从而使栽培番茄品种具有耐盐性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro Screening of Salt Tolerent Genotypes in Tomato (Solanum lycopersicum L.)
Soil salinity is one of the most important abiotic stress that limit crop production. Tomato (Solanum lycopersicum L.) is moderately tolerant to salinity and is typically cultivated in regions that are exposed to soil salinization. The aim of the study was to characterize phenotype response to salt stress under in vitro conditions of fourteen tomato genotypes. The shortest root length was observed entries BD-7260 at 250 mM. Longer roots (11.6 cm) were developed by the plants from the solutions containing 50 mM NaCl in entries BD-7302. NaCl concentration in the medium significantly affected the root length and plant weight of tomato. Genotypic distribution of weight LS means revealed that genotypes BARI-2 and the Line BD-7292 is the highest performed and the Line BD-7762 is the lowest performed. These findings indicated some salt tolerant tomato genotypes which will be promising for future hybridization program. Analysis of novel genes as well as some previously identified genes such as PIPs, LTPs, AGPs, PRPs, GRPs etc. which showed protective roles in different abiotic stresses to other crops is quiet necessary and the presence and expression pattern of those genes in these screened genotypes of tomato will provide powerful information for over-expression of those genes in transgenic plants those will confer salt tolerances to the cultivated tomato varieties.
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