T和B细胞缺陷Rag1 -/-突变斑马鱼的保护性免疫相关转录组变化

Aparna Krishnavajhala, Preeti J. Muire, L. Hanson, H. Wan, F. McCarthy, A. Zhou, L. Petrie‐Hanson
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引用次数: 1

摘要

为了阐明T细胞和B细胞缺陷的rag1 -/-突变斑马鱼保护性免疫的基础,我们对rag1 -/-突变斑马鱼在初次应答48小时和二次应答48小时后的15,617个基因进行了微阵列分析。初次暴露后,最大的表达倍数差异(3.8至4.95)是血清淀粉样蛋白A、趋化因子CCL-C5a (CCL-19a)、信号换能器和转录激活因子(STAT) 1b、干扰素调节因子11和黏液病毒抗性A的基因。这些基因的强诱导表明,T细胞和B细胞缺陷突变斑马鱼的初次免疫反应和先天免疫细胞没有受损。细菌再暴露后,趋化因子CCL-C5a (CCL-19a)、肌肥大素、骨形态发生蛋白4和松弛素3a的表达差异最大(2 - 3倍)。这些基因参与免疫反应和细胞增殖。细胞受体激活和信号转导、细胞增殖和细胞毒性功能的基因也上调。这些发现表明受体的激活和细胞群的扩增。48 hpi时ifnγ表达增加与原发性和继发性免疫反应相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1 -/- Mutant Zebrafish
To elucidate the basis of protective immunity in T and B cell deficient rag1 -/- mutant zebrafish, we conducted microarray analysis of 15,617 genes from rag1 -/- mutant zebrafish 48 hours after a primary response and 48 hours after a secondary response. Following primary exposure, the highest fold expression differences (3.8 to 4.95) were genes for serum amyloid A, chemokine CCL-C5a (CCL-19a), signal transducer and activator of transcription (STAT) 1b, interferon regulatory factor 11, and myxovirus resistance A. Strong induction of these genes demonstrated that primary immune responses and innate immune cells were not impaired in T and B cell deficient mutant zebrafish. Following bacterial re-exposure, the highest fold expression differences (2 to 3 fold) were in chemokine CCL-C5a (CCL-19a), myomegalin, bone morphogenetic protein 4, and relaxin 3a. These genes are involved in the immune response and cell proliferation. Genes for cell receptor activation and signal transduction, cell proliferation and cytotoxic functions were also up-regulated. These findings suggest receptor activation and expansion of a cell population. Increased ifnγ expression at 48 hpi was associated with both primary and secondary immune responses.
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