鹰嘴豆乙酰羟基酸合成酶基因的单核苷酸多态性:分子遗传分析

N. Volkova, H. Slishchuk, V. Sichkar, O. Zakharova
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引用次数: 0

摘要

的目标。鹰嘴豆AHAS基因分子标记分析。为实现这一目标,确定了鹰嘴豆AHAS基因SNP标记的研究与开发、鹰嘴豆品种和样品的标记基因分型。方法。CTAB法分离纯化DNA,实时聚合酶链反应。结果。鹰嘴豆AHAS1基因KASP基因特异性发现:与晶状体和玉米样品均未观察到DNA扩增。3个乌克兰鹰嘴豆品种和28个ICRISAT收集样本仅检测到野生型c等位基因。作者拥有的SNP标记对58份鹰嘴豆样品进行基因分型,结果表明样品内AHAS1基因为纯合子,未检测到突变的T等位基因。结论:31份鹰嘴豆样品经KASP基因分型鉴定含有野生型c等位基因,该等位基因与咪唑啉类除草剂敏感性有关。58份鹰嘴豆样本含有c等位基因,通过作者自己的SNP标记进行基因分型。与耐药缺失相关的突变体t等位基因表明所研究样品对咪唑类除草剂的耐受性较低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Single Nucleotide Polymorphism of the chickpea gene encoding acetohydroxyacid synthase: molecular-genetical analysis
Aim. Chickpea AHAS gene molecular-marker analysis. To achieve this aim following tasks were set: chickpea AHAS gene SNP markers research and development, chickpea varieties and samples genotyping by the markers. Methods. CTAB method of DNA isolation and purification, real-time polymerase chain reaction. Results. Chickpea AHAS1 gene KASP gene specificity was found: there were no DNA amplification with lens and maize samples observed. Three Ukrainian chickpea varieties and 28 ICRISAT collection samples KASP genotyping detected only wild-type C-allele. Authors own SNP markers 58 chickpea samples genotyping showed that AHAS1 gene within samples was homozygous, no mutant T allele was detected. Conclusions. 31 chickpea samples contained wild-type C-allele by KASP genotyping, which is associated with imidazoline herbicide susceptibility. 58 chickpea samples contained C-allele by authors own SNP markers genotyping. Mutant T-allele that is associated with tolerance absence indicates researched samples imidazole herbicide tolerance low level.
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