视网膜变性模型中金属的多模态成像为眼部疾病提供信息

Analytica Pub Date : 2023-07-01 DOI:10.3390/analytica4030021
Joshua Millar, L. Gibbons, C. Costa, Ella Schneider, J. von Gerichten, Michael C. Bailey, Susan Campbell, C. Duckett, Sarah L. Doyle, L. Cole
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引用次数: 0

摘要

金属组已参与眼组织病理研究数十年;然而,随着技术的进步,可以从单个组织切片中确定更多的信息,这在以前是不可能的。在此,互补技术的演示已被用来描述野生型(WT)和视紫红质(Rho−/−)眼部组织中必需金属的分布和浓度。所描述的多模态方法是采用多方面分析方法时可以产生的互补数据集的一个例子。通过激光烧蚀电感耦合等离子体质谱(LA-ICP-MS)在WT和Rho−/−组织中观察到铜和锌的异质分布,并通过粒子诱导x射线发射(PIXE)分析了ICP-MS分析中众所周知的微量元素(磷、硫、氯、钾、钙、铁和铝)的分布。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multimodal Imaging of Metals in a Retinal Degeneration Model to Inform on Ocular Disease
The metallome has been involved in the pathological investigation into ocular tissue for decades; however, as technologies advance, more information can be ascertained from individual tissue sections that were not previously possible. Herein, a demonstration of complementary techniques has been utilized to describe the distribution and concentrations of essential metals in both wildtype (WT) and rhodopsin (Rho−/−) ocular tissues. The multimodal approach described is an example of complementary datasets that can be produced when employing a multifaceted analytical approach. Heterogenous distributions of copper and zinc were observable within both WT and Rho−/− tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), and the distributions of further trace elements notoriously problematic for ICP-MS analysis (phosphorous, Sulfur, chlorine, potassium, calcium, iron, and aluminum) were analysed by particle-induced X-ray emission (PIXE).
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CiteScore
1.80
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