何首乌和薰衣草精油对耐多药鲍曼不动杆菌外排泵表型检测的协同作用

Q3 Medicine

Reviews in Medical Microbiology Pub Date : 2020-07-08 DOI:10.1097/MRM.0000000000000229

Parisa Agahi, Masoumeh Mahdavi-Ourtakand, F. Noorbakhsh

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引用次数: 0

摘要

背景:鲍曼不动杆菌感染已成为世界范围内医院获得性感染的主要原因，并可迅速对抗生素产生耐药性。鲍曼不动杆菌耐多药机制多种多样，外排泵是其中最重要的机制。本研究的目的是评价扎连和薰衣草精油对耐多药鲍曼不动杆菌外排泵表型检测的协同作用。方法:从临床标本中收集鲍曼不动杆菌55株，采用Kirby-Bauer纸片扩散法分离出耐多药菌株。采用气相色谱-质谱联用技术对何首乌挥发油进行了提取和分析。评价精油对MDR鲍曼不动杆菌的最低抑菌浓度和抑菌分数浓度指数，以确定其协同作用。采用溴化乙啶-琼脂轮盘法研究了耐多药菌株外排泵的活性，并评价了精油对外排泵活性的抑制作用。结果:本研究结果表明，多花莲挥发油和石竹挥发油对耐多药鲍曼不动杆菌具有加抑作用。采用侧翻法对外排泵进行了研究，结果表明，10%的菌株具有活性外排泵，在多花草和花楸精油1/2的最低抑菌浓度协同作用后，外排泵活性被抑制。结论:多花连珠草精油与石竹连珠草精油联合使用对耐多药鲍曼不动杆菌具有叠加性抑菌作用。还发现这些化合物作为外排泵的抑制剂，也可用于抑制MDR鲍曼不动杆菌的分离。

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Synergistic effect of Zataria multiflora and Lavandula stoechas essential oils on phenotype detection of efflux pump against multidrug-resistant Acinetobacter baumannii isolates

Background: Acinetobacter baumannii infections have become a major cause of hospital-acquired infections worldwide and can rapidly develop resistance to antibiotics. There are various mechanisms for multidrug resistance in the A. baumannii and efflux pumps is the most important among those. The purpose of this study is to evaluate the synergistic effect of Zataria multiflora and Lavandula stoechas essential oils on phenotype detection of efflux pump against multidrug-resistant A. baumannii isolates. Methods: A total of 55 A. baumannii strains were collected from clinical specimens and those with multidrug resistant (MDR) has been isolated by using the Kirby-Bauer disk diffusion method. Z. multiflora and L. stoechas essential oils were extracted and analyzed by gas chromatography–mass spectrometry. The minimum inhibitory concentration for antimicrobial activity of essential oils against MDR A. baumannii strains and fractional inhibitory concentration index was evaluated to determine their synergistic effect. The activity of efflux pumps of MDR strains was investigated by the ethidium bromide-agar cartwheel method and the inhibitory effect of essential oils on the efflux pumps activity was evaluated. Results: The results of this study showed that Z. multiflora and L. stoechas essential oils had additive antibacterial effects against MDR A. baumannii strains. The study of efflux pumps by cartwheel method showed that 10% of the strains had active efflux pump, after the effect of 1/2 minimum inhibitory concentration synergistic of Z. multiflora and L. stoechas essential oils, the activity of efflux pumps was inhibited. Conclusion: The combination of Z. multiflora and L. stoechas essential oils against MDR A. baumannii strains were additive antibacterial effects. It was also found that these compounds, as inhibitors of efflux pumps, could also be used to suppress MDR A. baumannii isolated.

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来源期刊

Reviews in Medical Microbiology 生物-微生物学

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Reviews in Medical Microbiology is a quarterly review journal which provides a balanced coverage of the whole field of medical microbiology. The Journal publishes state-of-the art reviews, mini-reviews, case presentations and original research from on-going research of the latest developments and techniques in medical microbiology, virology, mycology, parasitology, clinical microbiology, and hospital infection. In addition, PhD-Review - a platform for young researchers, and biographical Bio-Sketch articles are also considered. Reviews are concise, authoritative, and readable synthesis of the latest information on its subject, and references are limited to the fifty key sources for full reviews and twenty for mini-reviews. Reviews in Medical Microbiology is the perfect way for both qualified and trainee microbiologists, and researchers and clinicians with an interest in microbiology, to stay fully informed of the latest developments in medical microbiology. The journal is a valuable resource for educational and teaching purposes.