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E-Journal Menara Perkebunan Pub Date : 2022-04-28 DOI:10.22302/iribb.jur.mp.v90i1.483

Fitrianti Fitrianti, Loekas Soesanto, E. Mugiastuti, Murti Wisnu Ragil Sastyawan, Abdul Manan

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引用次数: 0

摘要

炭疽病是可可中的一种重要病害。本研究旨在确定从3株荧光假单胞菌分离得到的次生代谢物对可可叶片炭疽病的控制效果及其对可可植株生长的影响。该研究在日惹市Gunung Kidul Regency Patuk区Putat村的一个小农可可种植园进行，为期四个月。采用随机区组设计，设4个重复，共7个处理，分别为对照、荧光假单胞菌P60、荧光假单胞菌P20、荧光假单胞菌P8、荧光假单胞菌P60 + P20、荧光假单胞菌P60 + P8和荧光假单胞菌P20 + P8。观察的变量包括病害强度、侵染率、健康芽数和定性酚类化合物。结果表明，荧光假单胞菌的次生代谢物P60、P20和P8单独或联合使用对病害强度的抑制作用为42.01-54.50%。荧光假单胞菌P60、P20、P8、荧光假单胞菌P60+P20、荧光假单胞菌P60+P8、荧光假单胞菌P20+P8的代谢物继发感染率为0.23;0.25;0.26;0.26;0.31;和0.24单位/天。荧光假单胞菌P60的次生代谢产物使健康芽数增加67.44%。荧光假单胞菌P60的次生代谢产物增加了可可叶中的酚类化合物(单宁、皂苷和糖苷)。[关键词]叶片炭疽病，炭疽菌，可可，次生代谢物，荧光假单胞菌]摘要:炭疽菌，炭疽菌，炭疽菌，炭疽菌，炭疽菌，炭疽菌。Penelitian bertujuan mengkaji keefektifan代谢在分离的荧光假单胞菌(Pseudomonas fluorescens untuk mengendalikan penyakit antraknosa pada dadaan kakao)下，serta pengaruhnya terhadap pertumbuhan tanaman kakao。Penelitian dilaksanakan di perkebunan kakao rakyat, Desa puat, Kecamatan Patuk, Kabupaten Gunung Kidul，日惹selama empat bulan。Penelitian menggunakan rancangan acak kelompok untuk menguji 7 perlakuan yang diulang 4 kali, perlakuan yang diulang adalah对照，应用荧光假单胞菌P60、荧光假单胞菌P20、荧光假单胞菌P8、红木假单胞菌P60+P20、荧光假单胞菌P60+P8、丹荧光假单胞菌P20+P8代谢组。变量pengamatan meliputi intensitas penyakit, laju infeksi, jumlah tunas sehat, dan kandungan senyawa fenol secara quality。荧光假单胞菌P60, P20，和P8的代谢物研究[j] .中国生物医学工程学报，42(1):554 - 554,50%。荧光假单胞菌P60、荧光假单胞菌P20、荧光假单胞菌P8、红豆假单胞菌P60+P20、荧光假单胞菌P60+P8和荧光假单胞菌P20+P8代谢研究[0,23];0, 25;0, 26日;0, 26日;0, 31日;丹0,24单位/哈里。荧光假单胞菌(P. fluorescens P60 meningkatkan jumlah tunas)对代谢的影响为67,44%。Perlakuan P. fluorescens P60 meningkatkan senyawa fenol(皂苷，单宁，丹利柯西达)，paadan, kakao。[Kata kunci: antraknosa daun，炭疽菌gloeosporoides, kakao, metabolit sekunder, Pseudomonas fluorescens]

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Aplikasi metabolit sekunder dari tiga isolat Pseudomonas fluorescens untuk mengendalikan penyakit antraknosa pada daun kakao

Anthracnose caused by Colletotrichum gloeosporoides is an important disease in cocoa. This research aimed to determine the effectiveness of secondary metabolites derived from three isolates of Pseudomonas fluorescens to control cocoa leaves anthracnose, and their influence on the growth of cocoa plants. The research was conducted at a smallholder cocoa plantation in Putat Village, Patuk District, Gunung Kidul Regency, Yogyakarta for four months. A randomized block design was used with four replicates and seven treatments consisted of control, application of secondary metabolites from P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, combination P. fluorescens P60 + P20, P. fluorescens P60 + P8, and P. fluorescens P20 + P8. Variables observed were disease intensity, infection rate, number of healthy shoots and qualitative phenolic compound. Resultsof the research showed that the secondary metabolites of P. fluorescens P60, P20 and P8 alone or in combination suppressed the disease intensity by42.01-54.50%. The infection rate caused by metabolite secondary of P. fluorescens P60, P20, P8, P. fluorescens P60+P20, P. fluorescens P60+P8,and P. fluorescens P20+P8 was 0.23; 0.25; 0.26; 0.26; 0.31; and 0.24 units/day, respectively. The secondary metabolites of P. fluorescens P60 increased the number of healthy shoots by 67.44 %. The secondary metabolites of P. fluorescens P60 increased phenolic compounds (tannin, saponin, and glycosides) in cocoa leaves.[Keyword: leaves anthracnose, Colletotrichum gloeosporoides, cocoa, secondary metabolites, Pseudomonas fluorescens] AbstrakAntraknosa yang disebabkan oleh Colletotrichum gloeosporioides merupakan penyakit penting pada tanaman kakao. Penelitian bertujuan mengkaji keefektifan metabolit sekunder dari tiga isolat Pseudomonas fluorescens untuk mengendalikan penyakit antraknosa pada daun kakao, serta pengaruhnya terhadap pertumbuhan tanaman kakao. Penelitian dilaksanakan di perkebunan kakao rakyat, Desa Putat, Kecamatan Patuk, Kabupaten Gunung Kidul, Yogyakarta selama empat bulan. Penelitian menggunakan rancangan acak kelompok untuk menguji 7 perlakuan yang diulang 4 kali, perlakuan yang diuji adalah kontrol, aplikasi metabolit sekunder P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, kombinasi P. fluorescens P60+P20, P. fluorescens P60+P8, dan P. fluorescens P20+P8. Variabel pengamatan meliputi intensitas penyakit, laju infeksi, jumlah tunas sehat, dan kandungan senyawa fenol secara kualitatif. Hasil penelitian menunjukkan bahwa perlakuan metabolit sekunder P. fluorescens P60, P20, dan P8 secara tunggal dan gabungan mampu menekan intensitas penyakit berkisar 42,01-54,50%. Laju infeksi perlakuan metabolit sekunder P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, kombinasi P. fluorescens P60+P20, P. fluorescens P60+P8 dan P. fluorescens P20+P8 berturut-turut 0,23; 0,25; 0,26; 0,26; 0,31; dan 0,24 unit/hari. Metabolit sekunder P. fluorescens P60 meningkatkan jumlah tunas sehat sebesar 67,44%. Perlakuan P. fluorescens P60 meningkatkan senyawa fenol (saponin, tanin, dan glikosida) pada daun kakao.[Kata kunci: antraknosa daun, Colletotrichum gloeosporoides, kakao, metabolit sekunder, Pseudomonas fluorescens]

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E-Journal Menara Perkebunan

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