{"title":"用RAPD-PCR方法分析了世界各地相似雷家龙种群的基因组多样性。","authors":"M. Hahn, P. Burrows, D. Wright","doi":"10.1163/004625996X00036","DOIUrl":null,"url":null,"abstract":"DNA extracts from thirteen populations of Radoplaolus similis, collected previously from roots of various hosts at different locations around the world, and one population each of R. bridgei and R. citri, both collected in Indonesia, were compared using the polymerase chain reaction based method of random amplified polymorphic DNA (RAPD-PCR). Hierarchical cluster analysis was applied to the data obtained from RAPD profiles. This arranged the fifteen populations into two clusters. It also highlighted the two populations of R. citri and R. bridgei which were genomically distinct from R. similis. The results are discussed in the context of the genomic divergence of the species R. similis and the genus Radopholus.","PeriodicalId":18988,"journal":{"name":"Nematologica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"14","resultStr":"{\"title\":\"Genomic diversity between Radopholus similis populations from around the world detected by RAPD-PCR analysis.\",\"authors\":\"M. Hahn, P. Burrows, D. Wright\",\"doi\":\"10.1163/004625996X00036\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"DNA extracts from thirteen populations of Radoplaolus similis, collected previously from roots of various hosts at different locations around the world, and one population each of R. bridgei and R. citri, both collected in Indonesia, were compared using the polymerase chain reaction based method of random amplified polymorphic DNA (RAPD-PCR). Hierarchical cluster analysis was applied to the data obtained from RAPD profiles. This arranged the fifteen populations into two clusters. It also highlighted the two populations of R. citri and R. bridgei which were genomically distinct from R. similis. The results are discussed in the context of the genomic divergence of the species R. similis and the genus Radopholus.\",\"PeriodicalId\":18988,\"journal\":{\"name\":\"Nematologica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nematologica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1163/004625996X00036\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nematologica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1163/004625996X00036","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 14
摘要
采用基于随机扩增多态性DNA (RAPD-PCR)的聚合酶链反应方法，比较了先前从世界各地不同寄主根系中采集的13个相似Radoplaolus (Radoplaolus similis)居群，以及在印度尼西亚采集的bridgei和R. citri各1个居群的DNA提取物。采用层次聚类分析方法对RAPD剖面数据进行分析。这将15个种群分成了两个集群。同时还强调了柑橘r.r e citri和桥桥r.r e bridgei两个居群与相似r.r e similis在基因组上的差异。这些结果是在物种相似和Radopholus属的基因组差异的背景下讨论的。
Genomic diversity between Radopholus similis populations from around the world detected by RAPD-PCR analysis.
DNA extracts from thirteen populations of Radoplaolus similis, collected previously from roots of various hosts at different locations around the world, and one population each of R. bridgei and R. citri, both collected in Indonesia, were compared using the polymerase chain reaction based method of random amplified polymorphic DNA (RAPD-PCR). Hierarchical cluster analysis was applied to the data obtained from RAPD profiles. This arranged the fifteen populations into two clusters. It also highlighted the two populations of R. citri and R. bridgei which were genomically distinct from R. similis. The results are discussed in the context of the genomic divergence of the species R. similis and the genus Radopholus.
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