雷帕霉素对萼花臂尾轮虫寿命及TOR和S6K表达的影响

IF 1.3 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Aquatic Biology Pub Date : 2017-05-15 DOI:10.3354/AB00673

Huan-Zhi Xu, Lulu Liu, Yuqi Su, Ye Liang, Jiaxin Yang

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引用次数: 2

摘要

雷帕霉素的机制靶点(mTOR)协调了从翻译到自噬的复杂信号通路，不仅是生长和增殖的关键调节因子，而且是代谢和衰老的关键调节因子。mTOR对许多环境和内分泌刺激敏感。以雷帕霉素为外源抑制剂，研究TOR信号通路对萼花臂轮虫衰老和繁殖的影响。结果表明，与对照组相比，2 μM和4 μM雷帕霉素分别使萼花b的寿命延长了15%和22% (p < 0.05)。雷帕霉素在2 μM和4 μM时显著延迟了繁殖高峰(p < 0.01)，但繁殖前后期与对照组无显著差异(p > 0.05)。从萼花表达的序列标签中获得了编码TOR 375 bp和S6激酶(S6K) 951 bp的部分cdna。推测得到的花萼B. calyciflora cdna序列与人类同源物的同源性分别为TOR的58%和S6K的68%。不同雷帕霉素浓度(0.5、1、2、4、8和16 μM)和处理时间(对照、12、24、36和48 h)均可上调或下调TOR和S6K mRNA的表达。结果表明，TOR抑制对轮虫寿命的延长起叠加作用，上调和下调同时影响生殖和基因表达。

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Effects of rapamycin on life span and on expression of TOR and S6K in Brachionus calyciflorus (Rotifera)

The mechanistic target of rapamycin (mTOR) coordinates a complex signal pathway from translation to autophagy that is a key regulator of not only growth and proliferation but also metabolism and aging. mTOR is sensitive to many environmental and endocrine stimuli. We investigated the influence of TOR signaling on aging and reproduction of the rotifer Brachionus calyciflorus using rapamycin as an exogenous inhibitor. We found that 2 and 4 μM rapamycin extended B. calyciflorus life span by 15 and 22%, respectively compared with controls (p < 0.05). The reproductive peak was significantly delayed by rapamycin at 2 and 4 μM (p < 0.01), but the preand post-reproduction periods were not significantly different from controls (p > 0.05). Partial cDNAs coding 375 bp for TOR and 951 bp for S6 kinase (S6K) were obtained from B. calyciflorus expressed sequence tags. The identities of the deduced amino acid sequences of B. calyciflorus cDNAs to their human orthologs were 58% for TOR and 68% for S6K. TOR and S6K mRNA expression were upor down-regulated by different rapamycin concentrations (0.5, 1, 2, 4, 8, and 16 μM) and treatment intervals (control, 12, 24, 36, and 48 h). The results indicated that TOR inhibition acted additively to extend rotifer life span, with upand down-regulation simultaneously impacting reproduction and gene expression.

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来源期刊

Aquatic Biology 生物-海洋与淡水生物学

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： AB publishes rigorously refereed and carefully selected Feature Articles, Research Articles, Reviews and Notes, as well as Comments/Reply Comments (for details see MEPS 228:1), Theme Sections, Opinion Pieces (previously called ''As I See It'') (for details consult the Guidelines for Authors) concerned with the biology, physiology, biochemistry and genetics (including the ’omics‘) of all aquatic organisms under laboratory and field conditions, and at all levels of organisation and investigation. Areas covered include: -Biological aspects of biota: Evolution and speciation; life histories; biodiversity, biogeography and phylogeography; population genetics; biological connectedness between marine and freshwater biota; paleobiology of aquatic environments; invasive species. -Biochemical and physiological aspects of aquatic life; synthesis and conversion of organic matter (mechanisms of auto- and heterotrophy, digestion, respiration, nutrition); thermo-, ion, osmo- and volume-regulation; stress and stress resistance; metabolism and energy budgets; non-genetic and genetic adaptation. -Species interactions: Environment–organism and organism–organism interrelationships; predation: defenses (physical and chemical); symbioses. -Molecular biology of aquatic life. -Behavior: Orientation in space and time; migrations; feeding and reproductive behavior; agonistic behavior. -Toxicology and water-quality effects on organisms; anthropogenic impacts on aquatic biota (e.g. pollution, fisheries); stream regulation and restoration. -Theoretical biology: mathematical modelling of biological processes and species interactions. -Methodology and equipment employed in aquatic biological research; underwater exploration and experimentation. -Exploitation of aquatic biota: Fisheries; cultivation of aquatic organisms: use, management, protection and conservation of living aquatic resources. -Reproduction and development in marine, brackish and freshwater organisms