玛琅州立大学河流中超级细菌的遗传鉴定

Reeno Al Hikmatus Sholekah, D. Listyorini
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引用次数: 0

摘要

超级细菌会恢复为对抗生素有抗药性的菌株。这些细菌可能会在疾病治疗和环境中造成一些问题。本研究的目的是确定在流经玛琅州立大学校园区的河流中是否存在超级细菌。选择阿莫西林、四环素和氯霉素检测可能的细菌耐药性。从一个确定的地点采集水样,在添加抗生素和不添加抗生素的培养基上立即将水样扩散到Luria Bertani琼脂上。采用16S rRNA基因DNA条形码法进行分子鉴定,并进行形态学和革兰氏染色分析。共分离出革兰氏阴性菌落16株,分别为杆菌、双杆菌、双球菌和双球菌。8个耐药菌落的遗传鉴定使我们认为分离物可能属于气单胞菌、志贺氏菌和芽孢杆菌。为了更清楚地了解这些耐药分离株的正确分类位置,仍需要进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genetic Identification of Superbugs from River Streams in State University of Malang
Superbugs revert to bacterial strains, which exhibit resistance to antibiotics. These bacteria could cause some problems in disease treatment and the environment. This research aimed of this study was to identify the presence of superbugs in the river stream that flows through Malang State University campus area. Amoxicillin, tetracycline, and chloramphenicol were selected to test the possible bacterial resistance. Water samples taken from a decided spot were spread over Luria Bertani’s agar on both antibiotic supplemented and antibiotics-free media with no delay. Molecular identification was carried out using 16S rRNA gene DNA barcoding approach, completed with morphological and Gram staining analyses. A total of 16 isolates of gram-negative colonies were found in the form of bacilli, diplobacilli, cocci, and diplococci. The genetic identification of eight resistant colonies led us to suggest that the isolates may belong to Aeromonas, Shigella, and Bacillus. Further studies are still required to get a clearer view of the correct taxonomical position of those resistant isolates.
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