Ricarda Torre, M. Freitas, E. Costa-Rama, H. Nouws, C. Delerue-Matos
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引用次数: 1
摘要
采用丝网印刷碳电极作为传感器，研制了一种测定食品样品中原肌球蛋白(一种主要的虾类过敏原)的电化学免疫传感器。单克隆抗体和多克隆抗体用于三明治型免疫分析。分析信号采用碱性磷酸酶标记的二抗和3-吲哚基磷酸/硝酸银底物电化学获得。总检测时间为2 h 50 min，原肌球蛋白的定量范围为2.5 ~ 20 ng mL−1，检测限为1.7 ng mL−1。该免疫传感器成功应用于商业食品的分析。
Tropomyosin Analysis in Foods Using an Electrochemical Immunosensing Approach
A screen-printed carbon electrode was used as the transducer for the development of an electrochemical immunosensor for the determination of tropomyosin (a major shrimp allergen) in food samples. Monoclonal and polyclonal antibodies were used in a sandwich-type immunoassay. The analytical signal was electrochemically obtained using an alkaline phosphatase-labelled secondary antibody and a 3-indoxyl phosphate/silver nitrate substrate. The total assay time was 2 h 50 min and allowed the quantification of tropomyosin between 2.5 and 20 ng mL−1, with a limit of detection of 1.7 ng mL−1 The immunosensor was successfully applied to the analysis of commercial food products.
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