肺炎链球菌的谷胱甘肽还原酶

IF 0.9 4区 生物学
Mwilye Sikanyika, D. Aragão, C. McDevitt, M. Maher
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引用次数: 1

摘要

来自肺炎链球菌的谷胱甘肽还原酶(GR)是一种在该细菌细胞质中催化氧化谷胱甘肽(GSSG)还原为其还原形式(GSH)的黄酶。维持细胞内谷胱甘肽池对于活性氧和氮的解毒以及细胞内对锌离子的金属耐受性至关重要。本文对肺炎链球菌GR (SpGR)进行过表达纯化,并以2.56 A的分辨率测定其晶体结构。SpGR总体结构与其他表征的gr相似,具有二聚体结构,在二聚体界面处包含反平行的β-片。这一观察结果,结合与其他GR酶的界面结构的比较,可以将这些酶分为三类。动力学特性分析表明,SpGR酶的Km(GSSG)值(231.2±24.7µM)明显高于其他GR酶。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Glutathione reductase from Streptococcus pneumoniae
The glutathione reductase (GR) from Streptococcus pneumoniae is a flavo­enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to its reduced form (GSH) in the cytoplasm of this bacterium. The maintenance of an intracellular pool of GSH is critical for the detoxification of reactive oxygen and nitrogen species and for intracellular metal tolerance to ions such as zinc. Here, S. pneumoniae GR (SpGR) was overexpressed and purified and its crystal structure determined at 2.56 A resolution. SpGR shows overall structural similarity to other characterized GRs, with a dimeric structure that includes an antiparallel β-sheet at the dimer interface. This observation, in conjunction with comparisons with the interface structures of other GR enzymes, allows the classification of these enzymes into three classes. Analyses of the kinetic properties of SpGR revealed a significantly higher value for Km(GSSG) (231.2 ± 24.7 µM) in comparison to other characterized GR enzymes.
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来源期刊
自引率
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审稿时长
2-4 weeks
期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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