湘茶芽孢杆菌的抑菌活性及单宁耐受性昆兹变种assamica (J.W. Mast)Kitam)。

Patthanasak Rungsirivanich, N. Thongwai
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引用次数: 4

摘要

从苗木Camellia sinensis (L.)的叶片中分离得到16株芽孢杆菌。昆兹变种assamica (J.W. Mast)),采自泰国清迈和法莱省的苗族花园。根据16S rRNA基因序列对地衣芽孢杆菌(B. licheniformis)、暹罗芽孢杆菌(B. siamensis)和龙舌兰芽孢杆菌(B. tequilensis) 3种细菌进行分离。暹罗亚芽孢杆菌ML122-2、ML123-1和ML124-1培养液对金黄色葡萄球菌ATCC 25923和耐甲氧西林金黄色葡萄球菌DMST 20625有抑制作用,地衣芽孢杆菌ML071-1、ML073-1、ML075-1和ML076-2对蜡样芽孢杆菌TISTR 687和金黄色葡萄球菌ATCC 25923有抑制作用,抑制值分别在242.4 363.6和265.2 340.9 AU/ml之间。此外,B. siamensis ML122-2对单宁的耐受性为1% (w/v)。因此,B. siamensis ML122-2、ML123-1和ML124-1以及B. licheniformis ML071-1、ML073-1、ML075-1和ML076-2可能参与了苗族发酵过程的生物控制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antibacterial Activity and Tannin Tolerance of Bacillus spp. Isolated from Leaves of Miang (Camellia sinensis (L.) Kuntze var. assamica (J.W. Mast.) Kitam.)
Sixteen Bacillus isolates were obtained from leaves of Miang plant (Camellia sinensis (L.) Kuntze var. assamica (J.W. Mast.) Kitam.) collected from Miang gardens in Chiang Mai and Phrae provinces of Thailand. All bacterial isolates were identified within 3 species including B. licheniformis, B. siamensis and B. tequilensis based on 16S rRNA gene sequence. The culture broth of B. siamensis ML122-2, ML123-1 and ML124-1 could inhibit growth of Staphylococcus aureus ATCC 25923 and methicillin resistant S. aureus DMST 20625 while B. licheniformis ML071-1, ML073-1, ML075-1 and ML076-2 could inhibit growth of B. cereus TISTR 687 and S. aureus ATCC 25923 with the inhibitory value ranging between 242.4 363.6 and 265.2 340.9 AU/ml, respectively. Moreover, B. siamensis ML122-2 could tolerate tannin, 1% (w/v). Accordingly, B. siamensis ML122-2, ML123-1 and ML124-1 and B. licheniformis ML071-1, ML073-1, ML075-1 and ML076-2 may involve in biological control of Miang fermentation process.
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