{"title":"用生物测定法分离菝葜果实中抗诱变因子。","authors":"S. Kaur, Saroj Arora, S. Kaur, Subodh Kumar","doi":"10.1615/JENVPATHTOXONCOL.V22.I1.70","DOIUrl":null,"url":null,"abstract":"In the course of our search for novel polyphenolic antimutagenic agents from medicinal plants, we examined water, acetone, and chloroform extracts of Terminalia bellerica for their antimutagenic potency using the Ames Salmonella/microsome assay. Acetone extract exhibited variable inhibitory activity of 65.6%, and 69.7% with 4-O-nitrophenylenediamine (NPD) and sodium azide, respectively (as direct-acting mutagens), and 81.4% with 2-aminofluorene (2AF) (an S9-dependent mutagen), in the preincubation mode of experimentation. Inhibition with chloroform and water extracts was rather insignificant. Studies are well underway to isolate and identify the active polyphenolic compounds from acetone extract, which could be used as effective chemopreventive agents in the future.","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"18","resultStr":"{\"title\":\"Bioassay-guided isolation of antimutagenic factors from fruits of Terminalia bellerica.\",\"authors\":\"S. Kaur, Saroj Arora, S. Kaur, Subodh Kumar\",\"doi\":\"10.1615/JENVPATHTOXONCOL.V22.I1.70\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In the course of our search for novel polyphenolic antimutagenic agents from medicinal plants, we examined water, acetone, and chloroform extracts of Terminalia bellerica for their antimutagenic potency using the Ames Salmonella/microsome assay. Acetone extract exhibited variable inhibitory activity of 65.6%, and 69.7% with 4-O-nitrophenylenediamine (NPD) and sodium azide, respectively (as direct-acting mutagens), and 81.4% with 2-aminofluorene (2AF) (an S9-dependent mutagen), in the preincubation mode of experimentation. Inhibition with chloroform and water extracts was rather insignificant. Studies are well underway to isolate and identify the active polyphenolic compounds from acetone extract, which could be used as effective chemopreventive agents in the future.\",\"PeriodicalId\":94332,\"journal\":{\"name\":\"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2003-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"18\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1615/JENVPATHTOXONCOL.V22.I1.70\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1615/JENVPATHTOXONCOL.V22.I1.70","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 18
摘要
在我们从药用植物中寻找新的多酚类抗诱变剂的过程中，我们使用Ames沙门氏菌/微粒体试验检测了Terminalia bellerica的水、丙酮和氯仿提取物的抗诱变效力。在实验的预孵育模式下，丙酮提取物对4- o -硝基苯二胺(NPD)和叠氮化钠的抑制活性分别为65.6%和69.7%，对2-氨基芴(2AF) (s9依赖性诱变剂)的抑制活性为81.4%。氯仿和水提取物的抑制作用不明显。从丙酮提取物中分离和鉴定活性多酚类化合物的研究正在进行中，这些活性多酚类化合物有望在未来成为有效的化学预防剂。
Bioassay-guided isolation of antimutagenic factors from fruits of Terminalia bellerica.
In the course of our search for novel polyphenolic antimutagenic agents from medicinal plants, we examined water, acetone, and chloroform extracts of Terminalia bellerica for their antimutagenic potency using the Ames Salmonella/microsome assay. Acetone extract exhibited variable inhibitory activity of 65.6%, and 69.7% with 4-O-nitrophenylenediamine (NPD) and sodium azide, respectively (as direct-acting mutagens), and 81.4% with 2-aminofluorene (2AF) (an S9-dependent mutagen), in the preincubation mode of experimentation. Inhibition with chloroform and water extracts was rather insignificant. Studies are well underway to isolate and identify the active polyphenolic compounds from acetone extract, which could be used as effective chemopreventive agents in the future.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
