碱性磷酸酶活性作为生物化学标志物在水毒理学研究中的应用

Q3 Environmental Science

International Journal of Aquatic Biology Pub Date : 2020-04-25 DOI:10.22034/IJAB.V8I2.880

M. Banaee

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引用次数: 9

摘要

碱性磷酸酶(ALP)是一种具有金属磷酸酶结构的糖蛋白，在碱性ph下催化生物分子酯的单磷酸酯水解。ALP活性是评估细胞膜生理健康、细胞生长、细胞凋亡和细胞迁移、细胞代谢状态、肝细胞功能和肝细胞解毒活性的有用生物指标。利用对硝基苯基磷酸盐底物(p-NPP)在波长为405 nm的比色法检测生物样品中的ALP活性。溶血:细胞溶血，尤指红细胞;性激素和皮质类固醇水平升高、生物感染和营养不良可对ALP活性产生不利影响。

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Alkaline phosphatase activity as a biochemical biomarker in aqua-toxicological studies

Alkaline phosphatase (ALP) is a glycoprotein with a metallophosphatase structure that catalyzes the hydrolysis of monophosphate esters of biomolecule esters at alkaline pH. ALP activity is a useful bioindicator to assess the physiological health of cellular membranes, cell growth, apoptosis and cell migration, cellular metabolic status, hepatocyte function, and detoxification activity in hepatocytes. ALP activity is detected in a colorimetric method using the para-nitrophenyl phosphate substrate (p-NPP) at a wavelength of 405 nm in biological samples. Cell hemolysis, especially erythrocytes; increased levels of sex hormones and corticosteroids, biological infections, and poor nutrition can adversely affect ALP activity.

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来源期刊

International Journal of Aquatic Biology Environmental Science-Ecology

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

10 weeks