马尾藻的植物化学筛选、高效薄层色谱分析及其抗氧化活性

P. Kumar
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引用次数: 0

摘要

目的:筛选马尾藻不同溶剂提取物(己烷、氯仿、丙酮、乙酸乙酯和甲醇)中的植物化学成分。对所选提取物进行了高效薄层色谱(HPTLC)分析和抗氧化活性测定。材料和方法:所有提取物采用Harborne描述的标准程序筛选植物化学物质。以正己烷:甲苯:氯仿:乙酸乙酯为合适的流动相,用红外光谱(FT-IR)和高效液相色谱(HPTLC)对提取物中所含的最大成分进行了官能团鉴定。hplc指纹图谱在254 nm和366 nm下显示出植物化学成分条带。采用CAMAG薄层扫描仪对薄层斑点进行扫描。采用清除DPPH自由基的方法测定乙酸乙酯提取物的抗氧化活性。结果:乙酸乙酯提取物中生物碱、酚类、甾体、皂苷、黄酮类、单宁等成分含量最高。乙酸乙酯提取物在254 nm处有7个不同Rf值的HPTLC谱带,在366 nm处有12个最大Rf值谱带。DPPH分析表明,白氏葡萄球菌是一种潜在的抗氧化剂。白螺旋体的IC50值为320.05µg/ml。结论:该研究结果提示白蜡多糖具有食用来源,其多种营养成分显示出潜在的抗氧化活性
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochemical screening and high-performance thin-layer chromatography profile of Sargassum wightii and its antioxidant activity
Objective: The present study was aimed to screening the phytochemical constituents present in various solvent extracts such as hexane, chloroform, acetone, ethyl acetate, and methanol of Sargassum wightii. Selected extract was subjected to high-performance thin-layer chromatography (HPTLC) profile and its antioxidant activity. Materials and Methods: All extracts were screening of phytochemicals using standard procedures as described by Harborne. The maximum constituents contained in extract were subjected to identify the functional groups using FT-IR and also subjected for HPTLC screening with suitable mobile phase as hexane:toluene:chloroform:ethyl acetate. The HPTLC fingerprint exhibited the bands of phytochemical constituents and was visualized under UV 254 nm and 366 nm. TLC spots were scanned by CAMAG TLC scanner. Anti-oxidant activity of ethyl acetate extract was determined by DPPH free-radical scavenging activity. Results: Ethyl acetate extract was contained maximum number of components such as alkaloids, phenols, steroids, saponins, flavonoids, and tannins. HPTLC chromatogram of Ethyl acetate extract recorded seven bands at 254 nm with different Rf values whereas at 366 nm, 12 bands were recorded with maximum Rf values. The DPPH assay indicates S. wightii as a potential antioxidant. IC50 value of S. wightii is 320.05 µg/ml. Conclusion: The results suggest that S. wightii has an edible source for many nutrients show potential antioxidant activity
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