优化分光光度法测定淀粉含量

Analytica Pub Date : 2022-10-26 DOI:10.3390/analytica3040027
Palina Bahdanovich, Kevin Axelrod, A. Khlystov, V. Samburova
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引用次数: 0

摘要

淀粉是一种在自然界中大量存在的多糖,在许多生物和环境过程中通常被用作能量来源和能量储存。当然,淀粉往往是微量的,这就需要对低浓度样品中的淀粉进行定量分析。现有的基于彩色直链糖-碘复合物分光光度法检测淀粉的研究缺乏对分析过程和重要参数的详细描述。本研究对该分光光度法进行了优化、测试,并将其应用于花粉、真菌、细菌、藻类等化学成分未知的大气生物气溶胶中淀粉含量的研究。考察了pH、碘溶液浓度、淀粉溶液稳定性等实验参数,并在590 nm处测定了方法检出限(MDL)和定量限(LOQ)。结果表明,在pH为6.0、碘试剂浓度为0.2%的条件下,同一淀粉浓度的分光光度测定信号最高。MDL为0.22 μg/mL,定量限为0.79 μg/mL。该优化方法已成功地在生物气溶胶上进行了测试,可用于测定低浓度样品中的淀粉含量。生物气溶胶中淀粉含量为0.45±0.05 μg/mg(细菌)~ 4.3±0.06 μg/mg(真菌)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimized Spectrophotometry Method for Starch Quantification
Starch is a polysaccharide that is abundantly found in nature and is generally used as an energy source and energy storage in many biological and environmental processes. Naturally, starch tends to be in miniscule amounts, creating a necessity for quantitative analysis of starch in low-concentration samples. Existing studies that are based on the spectrophotometric detection of starch using the colorful amylose–iodine complex lack a detailed description of the analytical procedure and important parameters. In the present study, this spectrophotometry method was optimized, tested, and applied to studying starch content of atmospheric bioaerosols such as pollen, fungi, bacteria, and algae, whose chemical composition is not well known. Different experimental parameters, including pH, iodine solution concentrations, and starch solution stability, were tested, and method detection limit (MDL) and limit of quantification (LOQ) were determined at 590 nm. It was found that the highest spectrophotometry signal for the same starch concentration occurs at pH 6.0, with an iodine reagent concentration of 0.2%. The MDL was determined to be 0.22 μg/mL, with an LOQ of 0.79 μg/mL. This optimized method was successfully tested on bioaerosols and can be used to determine starch content in low-concentration samples. Starch content in bioaerosols ranged from 0.45 ± 0.05 (in bacteria) to 4.3 ± 0.06 μg/mg (in fungi).
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CiteScore
1.80
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