毛毛紫荆叶提取物抗氧化总黄酮的细胞毒性研究

Nigerian Journal of Natural Products and Medicine Pub Date : 2009-09-03 DOI:10.4314/NJNPM.V12I1.45670

M. Aderogba, L. McGaw, A. Ogundaini, J. Eloff

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引用次数: 14

摘要

采用1,1 -二苯基-2-苦味酰肼(DPPH)活性引导分离方法分离了50%乙醇干燥毛毛紫荆叶提取物的抗氧化成分。采用加速梯度层析(AGC)和Sephadex LH-20柱层析分离得到山奈酚-7- o -鼠李糖苷(1)、山奈酚-3- o -葡萄糖苷(2)、槲皮素-3- o -葡萄糖苷(3)和槲皮素-3-核桃苷(4)四个黄酮醇苷类化合物。化合物1 ~ 3为首次从该植物中分离得到。化合物的结构通过波谱方法(1H NMR, 13C NMR和MS)确定。采用DPPH分光光度法测定分离得到的化合物的抗氧化活性。化合物3的抗氧化活性高于l -抗坏血酸，而化合物1和化合物4的抗氧化活性相近，化合物2的抗氧化活性较低。化合物2 ~ 4对牛皮肤细胞无细胞毒性，而山奈酚-7- 0 -鼠李糖苷(化合物1)对牛皮肤细胞有轻微的细胞毒性(LC50 = 116.58 μg/ml)

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Cytotoxicity Study Of Antioxidant Flavonoids From Bauhinia Tomentosa Leaf Extract

A 1, 1-diphenyl-2-picrylhydrazyl (DPPH) activity-guided fractionation procedure was used to isolate the antioxidant constituents of a 50% ethanol dried leaf extract of Bauhinia tomentosa. Four flavonol glycosides: kaempferol-7-O-rhamnoside (1), kaempferol-3-O-glucoside (2), quercetin-3-O-glucoside (3) and quercetin-3-Orutinoside (4) were isolated by accelerated gradient chromatography (AGC) and Sephadex LH-20 column chromatography. Compounds 1-3 are reported for the first time from this species. The structures of the compounds were established by spectroscopic methods (1H NMR, 13C NMR and MS). A DPPH spectrophotometric assay was employed to evaluate the antioxidant activity of the isolated compounds. Compound 3 had higher antioxidant activity than L-ascorbic acid while 1 and 4 similar and 2 lower activities. Compounds 2-4 were non-cytotoxic, but kaempferol-7-O-rhamnoside (compound 1) displayed slight cytotoxicity to bovine dermal cells (LC50 = 116.58 μg/ml) in the tetrazolium (MTT) based assay

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Nigerian Journal of Natural Products and Medicine

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