制备胶体几丁质用于几丁质酶产生微生物筛选的简化方法

N. Murthy, B. Bleakley
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引用次数: 82

摘要

研究了一种从廉价的蟹壳片中制备胶体甲壳素的简便高效方法。它改进了现有技术的一些步骤,为胶体甲壳素的制备提供了显著的省力和节省材料。在胶体几丁质制备中,除非最初的几丁质薄片被磨成细粉,否则以后很难从沉淀的胶体几丁质中分离出几丁质块。本文报道的改进技术包括使用简单的日常实验室材料从蟹壳薄片中提取胶体几丁质,而不需要将几丁质薄片磨成均匀的细尺寸。所获得的胶体几丁质的效用是通过用它在平板试验中筛选细胞外几丁质酶的生产者,并用Remazol Brilliant Blue (RBB)标记它进行几丁质酶试验。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Simplified Method of Preparing Colloidal Chitin Used For Screening of Chitinase- Producing Microorganisms
A simplified and efficient method of preparing colloidal chitin from inexpensive crab shell flakes was developed. It modifies some steps of existing techniques to provide significant saving in effort and materials for colloidal chitin preparation. In colloidal chitin preparation, unless the initial chitin flakes are ground to a fine powder, it becomes difficult to later separate the chitin chunks from the precipitated colloidal chitin. The modified technique reported here involves the use of simple everyday lab materials to extract colloidal chitin from crab shell flakes, without the need of powdering the chitin flakes to a uniformly fine size. Utility of the colloidal chitin obtained was shown by using it in plate assays to screen for extracellular chitinase producers, and labeling it with Remazol Brilliant Blue (RBB) for chitinase assays.
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