{"title":"体外和计算机研究评价槲皮素作为登革热病毒抗病毒药物的有效性","authors":"B. Dewi, E. Ratningpoeti, H. Desti, M. Angelina","doi":"10.1063/1.5139341","DOIUrl":null,"url":null,"abstract":"Dengue Fever (DF) in Indonesia has been a major health problem for the last 47 years, which keeps on rising until today. Quercitrin as one of the natural compounds, flavonol group and has been used in several types of research for their properties of anti-inflammation, anti-bacterial, anti-viral, and anti-fungi. The aim of this study was to explore the potency of quercitrin as an antiviral drug to dengue virus (DENV) in vitro and in silico. We used Focus assay, MTT assay, and docking analysis to determine IC50, CC50 and binding energy, respectively. The IC50, CC50 and Selectivity Index (SI) of quercitrin was 1.1 µg/ml, 38.8 µg/ml and 38 respectively. In silico study showed the binding energy between quercitrin and NS5 protein was −7,54 kkal/mol. The results obtained that Quercutrin as a good candidate for an antiviral drug to DENV in the future.Dengue Fever (DF) in Indonesia has been a major health problem for the last 47 years, which keeps on rising until today. Quercitrin as one of the natural compounds, flavonol group and has been used in several types of research for their properties of anti-inflammation, anti-bacterial, anti-viral, and anti-fungi. The aim of this study was to explore the potency of quercitrin as an antiviral drug to dengue virus (DENV) in vitro and in silico. We used Focus assay, MTT assay, and docking analysis to determine IC50, CC50 and binding energy, respectively. The IC50, CC50 and Selectivity Index (SI) of quercitrin was 1.1 µg/ml, 38.8 µg/ml and 38 respectively. In silico study showed the binding energy between quercitrin and NS5 protein was −7,54 kkal/mol. The results obtained that Quercutrin as a good candidate for an antiviral drug to DENV in the future.","PeriodicalId":22239,"journal":{"name":"THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019","volume":"32 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":"{\"title\":\"In vitro and in silico study to evaluate the effectiveness of quercitrin as antiviral drug to dengue virus\",\"authors\":\"B. Dewi, E. Ratningpoeti, H. Desti, M. Angelina\",\"doi\":\"10.1063/1.5139341\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Dengue Fever (DF) in Indonesia has been a major health problem for the last 47 years, which keeps on rising until today. Quercitrin as one of the natural compounds, flavonol group and has been used in several types of research for their properties of anti-inflammation, anti-bacterial, anti-viral, and anti-fungi. The aim of this study was to explore the potency of quercitrin as an antiviral drug to dengue virus (DENV) in vitro and in silico. We used Focus assay, MTT assay, and docking analysis to determine IC50, CC50 and binding energy, respectively. The IC50, CC50 and Selectivity Index (SI) of quercitrin was 1.1 µg/ml, 38.8 µg/ml and 38 respectively. In silico study showed the binding energy between quercitrin and NS5 protein was −7,54 kkal/mol. The results obtained that Quercutrin as a good candidate for an antiviral drug to DENV in the future.Dengue Fever (DF) in Indonesia has been a major health problem for the last 47 years, which keeps on rising until today. Quercitrin as one of the natural compounds, flavonol group and has been used in several types of research for their properties of anti-inflammation, anti-bacterial, anti-viral, and anti-fungi. The aim of this study was to explore the potency of quercitrin as an antiviral drug to dengue virus (DENV) in vitro and in silico. We used Focus assay, MTT assay, and docking analysis to determine IC50, CC50 and binding energy, respectively. The IC50, CC50 and Selectivity Index (SI) of quercitrin was 1.1 µg/ml, 38.8 µg/ml and 38 respectively. In silico study showed the binding energy between quercitrin and NS5 protein was −7,54 kkal/mol. 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In vitro and in silico study to evaluate the effectiveness of quercitrin as antiviral drug to dengue virus
Dengue Fever (DF) in Indonesia has been a major health problem for the last 47 years, which keeps on rising until today. Quercitrin as one of the natural compounds, flavonol group and has been used in several types of research for their properties of anti-inflammation, anti-bacterial, anti-viral, and anti-fungi. The aim of this study was to explore the potency of quercitrin as an antiviral drug to dengue virus (DENV) in vitro and in silico. We used Focus assay, MTT assay, and docking analysis to determine IC50, CC50 and binding energy, respectively. The IC50, CC50 and Selectivity Index (SI) of quercitrin was 1.1 µg/ml, 38.8 µg/ml and 38 respectively. In silico study showed the binding energy between quercitrin and NS5 protein was −7,54 kkal/mol. The results obtained that Quercutrin as a good candidate for an antiviral drug to DENV in the future.Dengue Fever (DF) in Indonesia has been a major health problem for the last 47 years, which keeps on rising until today. Quercitrin as one of the natural compounds, flavonol group and has been used in several types of research for their properties of anti-inflammation, anti-bacterial, anti-viral, and anti-fungi. The aim of this study was to explore the potency of quercitrin as an antiviral drug to dengue virus (DENV) in vitro and in silico. We used Focus assay, MTT assay, and docking analysis to determine IC50, CC50 and binding energy, respectively. The IC50, CC50 and Selectivity Index (SI) of quercitrin was 1.1 µg/ml, 38.8 µg/ml and 38 respectively. In silico study showed the binding energy between quercitrin and NS5 protein was −7,54 kkal/mol. The results obtained that Quercutrin as a good candidate for an antiviral drug to DENV in the future.
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