盐酸二甲双胍对雄激素敏感、LNCAP和雄激素不敏感的人前列腺癌细胞PC-3的体外抗增殖分析

Brittney V. Hinton, Dolapo Adedeji, G. Payne
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引用次数: 5

摘要

前列腺癌是世界范围内导致男性癌症相关死亡的疾病之一。二甲双胍是一种抗糖尿病药物,用于治疗II型糖尿病(DM2)已有20多年的历史。本研究采用3-[4,5-二甲基噻唑-2-酰基]-2,5-二苯基溴化四唑(MTT)法,研究盐酸二甲双胍对不同浓度(μM和mM)雄激素敏感、LNCAP和雄激素不敏感、PC-3人前列腺癌细胞的抑制增殖作用。盐酸二甲双胍对雄激素不敏感的前列腺癌细胞PC-3的细胞毒性比雄激素敏感的前列腺癌细胞强。对于两种细胞系,盐酸二甲双胍在0.1 mM浓度下表现出最佳的抗增殖活性,暴露120小时后细胞平均死亡率为60%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In-Vitro Antiproliferative Analysis of Metformin Hydrochloride on Androgen-Sensitive, LNCAP and Androgen-Insensitive, PC-3 Human Prostate Cancer Cell Lines
Prostate cancer is one of the diseases worldwide that causes cancer-related deaths in men. Metformin is an antidiabetic drug that has been in use for over two decades for the treatment of Type II Diabetes mellitus (DM2). The purpose of this study was to evaluate the anti-proliferative property of metformin hydrochloride on androgen-sensitive, LNCAP and androgen-insensitive, PC-3 human prostate cancer cell lines at different concentrations (μM and mM) using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Metformin hydrochloride displayed a stronger cytotoxicity on the androgen-insensitive PC-3 than on the androgen-sensitive human prostate cancer cell lines. For both cell lines, the antiproliferative activity of metformin hydrochloride was best displayed at 0.1 mM concentration with average cell death percentage of 60% after 120-hour exposure.
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