{"title":"高压液相色谱-二极管阵列检测(HPLC-DAD)法控制原甘草产品的质量","authors":"S. Chigome, M. Nindi, Nm Munkombwe","doi":"10.4314/NJNPM.V13I1.61584","DOIUrl":null,"url":null,"abstract":"Iridoid and phenylethanoid glycosides were used as compounds to monitor the quality of Devil’s Claw ( Harpagophytum procumbens ) based products in this investigation. A HPLC-DAD method was developed for the determination of the phenylethanoid glycoside isoacteoside and the iridoid glycoside harpagoside in commercially available Devil’s Claw products. The limits of detection (LOD) obtained were 1.08 μg/ml for harpagoside and 1.70 μg/ml for isoacteoside. Limits of quantification (LOQ) of harpagoside and isoacteoside were 3.59 μg/ml and 5.65 μg/ml respectively. The harpagoside and isoacteoside contents in the ethanol extracts of the commercial products were found to be in the range of 2.2 % - 3.9 % and 0.22 % - 0.50 % respectively. Keywords: Harpagophytum procumbens , phenylethanoid glycosides, iridoid glycosides, harpagoside, HPLC-DAD profiles","PeriodicalId":19356,"journal":{"name":"Nigerian Journal of Natural Products and Medicine","volume":"100 1","pages":"26-29"},"PeriodicalIF":0.0000,"publicationDate":"2010-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Quality control of Harpagophytum procumbens products using High Pressure Liquid Chromatography-Diode Array Detection (HPLC-DAD)\",\"authors\":\"S. Chigome, M. Nindi, Nm Munkombwe\",\"doi\":\"10.4314/NJNPM.V13I1.61584\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Iridoid and phenylethanoid glycosides were used as compounds to monitor the quality of Devil’s Claw ( Harpagophytum procumbens ) based products in this investigation. A HPLC-DAD method was developed for the determination of the phenylethanoid glycoside isoacteoside and the iridoid glycoside harpagoside in commercially available Devil’s Claw products. The limits of detection (LOD) obtained were 1.08 μg/ml for harpagoside and 1.70 μg/ml for isoacteoside. Limits of quantification (LOQ) of harpagoside and isoacteoside were 3.59 μg/ml and 5.65 μg/ml respectively. The harpagoside and isoacteoside contents in the ethanol extracts of the commercial products were found to be in the range of 2.2 % - 3.9 % and 0.22 % - 0.50 % respectively. Keywords: Harpagophytum procumbens , phenylethanoid glycosides, iridoid glycosides, harpagoside, HPLC-DAD profiles\",\"PeriodicalId\":19356,\"journal\":{\"name\":\"Nigerian Journal of Natural Products and Medicine\",\"volume\":\"100 1\",\"pages\":\"26-29\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-11-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nigerian Journal of Natural Products and Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4314/NJNPM.V13I1.61584\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nigerian Journal of Natural Products and Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4314/NJNPM.V13I1.61584","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Quality control of Harpagophytum procumbens products using High Pressure Liquid Chromatography-Diode Array Detection (HPLC-DAD)
Iridoid and phenylethanoid glycosides were used as compounds to monitor the quality of Devil’s Claw ( Harpagophytum procumbens ) based products in this investigation. A HPLC-DAD method was developed for the determination of the phenylethanoid glycoside isoacteoside and the iridoid glycoside harpagoside in commercially available Devil’s Claw products. The limits of detection (LOD) obtained were 1.08 μg/ml for harpagoside and 1.70 μg/ml for isoacteoside. Limits of quantification (LOQ) of harpagoside and isoacteoside were 3.59 μg/ml and 5.65 μg/ml respectively. The harpagoside and isoacteoside contents in the ethanol extracts of the commercial products were found to be in the range of 2.2 % - 3.9 % and 0.22 % - 0.50 % respectively. Keywords: Harpagophytum procumbens , phenylethanoid glycosides, iridoid glycosides, harpagoside, HPLC-DAD profiles
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