端粒酶逆转录酶（TERT）基因的两个共遗传 SNP 与伊拉克肺癌患者有关。

IF 2 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Medical Biochemistry Pub Date : 2023-10-27 DOI:10.5937/jomb0-41553

Zahraa K Lawi, Ahmed H Alkhammas, Malek Elerouri, Amara Ibtissem Ben, Mohammed Baqur S Al-Shuhaib

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引用次数: 0

摘要

背景：端粒酶逆转录酶（TERT）基因是与大多数恶性肿瘤相关的重要多态性位点。本研究评估了 TERT 基因与伊拉克非小细胞肺癌（NSCLC）之间的关系：方法：从总共 200 份血液样本中提取基因组 DNA 样本。设计了四个特异性 PCR 片段，以扩增 TERT 基因中的四个高频 SNPs：rs2735940、rs2736098、rs2736100 和 rs10069690。利用单链构象多态性（SSCP）和测序反应对扩增片段进行基因分型和验证。

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Two co-inherited SNPs of the telomerase reverse transcriptase (TERT) gene are associated with Iraqi patients with lung cancer.

Background: The telomerase reverse transcriptase (TERT) gene is essential polymorphic loci linked to most malignant tumors. This study assessed the association between the TERT gene and non-small cell lung carcinoma (NSCLC) in Iraq.

Methods: Genomic DNA samples were extracted from a total of 200 samples of blood. Four specific PCR fragments were designed to amplify four high-frequency rs2735940, rs2736098, rs2736100, and rs10069690 SNPs within the TERT gene. Single-strand conformation polymorphism (SSCP) followed by sequencing reactions were used for genotyping and validating the amplified fragments.

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来源期刊

Journal of Medical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.00

自引率

12.00%

发文量

审稿时长

>12 weeks

期刊介绍： The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly. The Journal publishes original scientific and specialized articles on all aspects of clinical and medical biochemistry, molecular medicine, clinical hematology and coagulation, clinical immunology and autoimmunity, clinical microbiology, virology, clinical genomics and molecular biology, genetic epidemiology, drug measurement, evaluation of diagnostic markers, new reagents and laboratory equipment, reference materials and methods, reference values, laboratory organization, automation, quality control, clinical metrology, all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.